On Thu, 6 Jan 2000, Richard Cartun wrote:

> I am interested in hearing what Histoneters are using for the detection
>of CD3+ cells in paraffin sections (pAb vs. mAb, digestion vs. HIER,
>etc.).  Thanks.
>
> R. Cartun
>
Hello Dr. Cartun,
I use Dako's LSAB2 kit, that's a 10 min incubation time for each of the
two reagents included in the kit. I pretreat it with BioGenex' citra
buffer for 15 minutes.  The CD3 I use is Dako's polyclonal cat.# A0452 and
I use it on the Dako autostainer.  I get fantastic results.

Dana Settembre
Immunohistochemistry Lab
Pathology Department
University Hospital
Newark,  New Jersey
USA