Hi Beth,

I do alot of BrDu stains on mouse and rat and so soaking the blocks to get
nice sections is necessary. I use ammonia water most of the time and still
get very good BrDU staining. Just be sure that you have a good retrievel if
they are paraffin sections. That will make a big difference on your
staining results.

Good luck,

Colleen Forster
U of MN, Dept. of Neurology

HistoScientific wrote:

> Dear Netters,
>
> Happy New Year!  We are serial-sectioning medaka fish for BrdU
> staining.  Generally, in order to section several sections at a time, we
> rub the block with ammonia water.  However, we are not sure whether or
> not the ammonia will have a negative affect on the BrdU staining.  Does
> anyone have any insight into these matters?  Thank you in advance.
>
> Sincerely,
>
> Beth Poole
> Histo-Scientific Research Labs.
> 107 Killmon Road
> Basye, VA  22810
> (540)856-2222
> fax: (540)856-2227
> histosci@shentel.net