We use DAKO's mAb to ALK-1 protein (M7195) at a dilution of 1:50 for a 30' minute incubation or 1:500 for overnight incubation with EnVision+/DAB+ detection.  DAKO's regular pH retrieval solution (steamer or waterbath) is used to improve immunoreactivity.  More importantly, please make sure that you have a good positive control.  Not all CD30+ anaplastic large cell lymphomas express the ALK-1 protein.  I have control tissue if you need it.

R. Cartun


>>> "Fessler, Jim" <jifess@pahosp.com> 01/11/01 08:37AM >>>
I am trying to get the Alk Protein monoclonal antibody from Dako to work
(with no success).  Any suggestions would be greatly appreciated. thanks