Re: Alk Protein Antibody
|From:||Richard Cartun <Rcartun@harthosp.org>|
We use DAKO's mAb to ALK-1 protein (M7195) at a dilution of 1:50 for a 30' minute incubation or 1:500 for overnight incubation with EnVision+/DAB+ detection. DAKO's regular pH retrieval solution (steamer or waterbath) is used to improve immunoreactivity. More importantly, please make sure that you have a good positive control. Not all CD30+ anaplastic large cell lymphomas express the ALK-1 protein. I have control tissue if you need it.
>>> "Fessler, Jim" <email@example.com> 01/11/01 08:37AM >>>
I am trying to get the Alk Protein monoclonal antibody from Dako to work
(with no success). Any suggestions would be greatly appreciated. thanks
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