Rich,

We use an almost identical procedure as written by Mr. Nocito, except we
make our retrieval solution, and have a slightly higher concentration of
CD3. We try for short incubation times to get the slides back to the
pathologists faster.

Neal


Dr. Cartun,
we are using a mouse monoclonal from Nova Castra.  We steam the slides
with
Dako's Target Retrieval solution for 20 minutes, with a 20 minute cool
down.
Our titer is 1:200.  We incubate at room temperature for 30 minutes each
in
the primary, link and label with a 5 minute incubation in DAB.  Hope
this
helps.

Joe Nocito, B.S., HT(ASCP)QIHC
Histology Supervisor
Christus Santa Rosa Hospitals
San Antonio, Texas

> -----Original Message-----
> From: Richard Cartun [SMTP:Rcartun@harthosp.org]
> Sent: Thursday, January 06, 2000 9:35 AM
> To:   Histonet@pathology.swmed.edu
> Subject:      CD3
>
> I am interested in hearing what Histoneters are using for the detection of
> CD3+ cells in paraffin sections (pAb vs. mAb, digestion vs. HIER, etc.).
> Thanks.
>
> R. Cartun
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