1.  frozen sections
2.  carbowax embedding


basically, to prevent shrinkage you are going to have to abstain from heat,
alcohol, and xylene.


rob

-----Original Message-----
From: Peter Poon [mailto:peterp@cafe.berkeley.edu]
Sent: January 10, 2000 11:52 AM
To: histonet
Subject: shrinkage in the dehydration of mouse brains


hi all, I have been working on the dehydration of mouse brains, but I have
been getting about a 50% shrinkage on overall volume. I have been using a
ethanol/isopropyl dehydration series. Does anyone have any ideas on how to
prevent shrinkage? Please help . Thanks


   __________________________________________
   \    Peter Poon                           \     _         ______ |
    \   http://cafe.berkeley.edu/~peterp      \  /   \___-=0`/|0`/__|
     \  (510)649-9654                          \_\          / | /    )
     /                                         /  `/-==__ _/__|/__ -|
    /                                         /   *             \ | |
   /                                         /                   (o)
   ------------------------------------------