RE: "Venetian Blind" Artefact

From:Alan Bright <>

Dear Stephanie,

Your "Venetian Blind" Artefact should be eliminated if you set your chamber
temperature to -20C or -25C and the object temperature to -8C to -12C.
Please let me know how this works out.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
PE29 6EU

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Web Site:

-----Original Message-----
From: Stephanie Moore []
Sent: 12 January 2001 15:23
To: HistoNet Server
Subject: "Venetian Blind" Artefact

I have recently experienced what I believe to be this artefact while slicing
sections of squirrel brain.  I know that this was not properly sunk and also
it was likely in too little volume of paraformaldehyde (4%) fixative.  The
who gave me the specimen is not very experienced, but wanted to slice it
My question is, does insufficient cryoprotection from sucrose cause
blinds?  I use a cryostat set at -20C chamber temperature and -18C object
temperature, and Tussue Tek OCT medium for embedding the specimen.

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