RE: "Venetian Blind" Artefact
|From:||Alan Bright <email@example.com>|
Your "Venetian Blind" Artefact should be eliminated if you set your chamber
temperature to -20C or -25C and the object temperature to -8C to -12C.
Please let me know how this works out.
Bright Instrument Co.Ltd.
St Margaret's Way
Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Web Site: www.brightinstruments.com
From: Stephanie Moore [mailto:firstname.lastname@example.org]
Sent: 12 January 2001 15:23
To: HistoNet Server
Subject: "Venetian Blind" Artefact
I have recently experienced what I believe to be this artefact while slicing
sections of squirrel brain. I know that this was not properly sunk and also
it was likely in too little volume of paraformaldehyde (4%) fixative. The
who gave me the specimen is not very experienced, but wanted to slice it
My question is, does insufficient cryoprotection from sucrose cause
blinds? I use a cryostat set at -20C chamber temperature and -18C object
temperature, and Tussue Tek OCT medium for embedding the specimen.
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