RE: "Venetian Blind" Artefact

From:Emma Carter <>

Was everything firmly locked into place? it sounds a bit like chattering
rather than insufficient fixing, though of course, this is just my

Emma Carter
Oxford BioMedica (UK)Ltd

> -----Original Message-----
> From:	Stephanie Moore 
> Sent:	Friday, January 12, 2001 3:23 PM
> To:	HistoNet Server
> Subject:	"Venetian Blind" Artefact
> I have recently experienced what I believe to be this artefact while
> slicing
> sections of squirrel brain.  I know that this was not properly sunk
> and also that
> it was likely in too little volume of paraformaldehyde (4%) fixative.
> The person
> who gave me the specimen is not very experienced, but wanted to slice
> it anyway.
> My question is, does insufficient cryoprotection from sucrose cause
> ventetian
> blinds?  I use a cryostat set at -20C chamber temperature and -18C
> object
> temperature, and Tussue Tek OCT medium for embedding the specimen.

<< Previous Message | Next Message >>