RE: "Venetian Blind" Artefact
|From:||Emma Carter <E.Carter@oxfordbiomedica.co.uk>|
Was everything firmly locked into place? it sounds a bit like chattering
rather than insufficient fixing, though of course, this is just my
Oxford BioMedica (UK)Ltd
> -----Original Message-----
> From: Stephanie Moore
> Sent: Friday, January 12, 2001 3:23 PM
> To: HistoNet Server
> Subject: "Venetian Blind" Artefact
> I have recently experienced what I believe to be this artefact while
> sections of squirrel brain. I know that this was not properly sunk
> and also that
> it was likely in too little volume of paraformaldehyde (4%) fixative.
> The person
> who gave me the specimen is not very experienced, but wanted to slice
> it anyway.
> My question is, does insufficient cryoprotection from sucrose cause
> blinds? I use a cryostat set at -20C chamber temperature and -18C
> temperature, and Tussue Tek OCT medium for embedding the specimen.
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