RE: double IHC/in situ

From:"Connolly, Brett M" <brett_connolly@merck.com>

Karen,

You may be interested in this article, J Histo Cyto 48(10): 1369-1375, 2000.

Brett


Brett M. Connolly, Ph.D.
Merck Research Laboratories
Department of Pharmacology
WP26A-3000
PO Box 4
West Point, PA 19486
Ph. 215-652-2501
FAX 215-652-2075
e-mail: brett_connolly@merck.com


> ----------
> From: 	Karen Larison[SMTP:larisonk@uoneuro.uoregon.edu]
> Sent: 	Thursday, January 11, 2001 3:02 PM
> To: 	HistoNet@Pathology.swmed.edu
> Subject: 	double IHC/in situ
> 
> Hello Histonetters,
> 
> Some of the researchers around here do in situ hybridization followed 
> by IHC.  However, the harsh conditions of the in situ hybridization 
> techniques destroys many of the antigenic sites of interest.  Does 
> anyone out there use antigen retrieval to recover antigenicity 
> following in situ techniques, or does anyone know of any references?
> 
> Karen Larison
> University of Oregon
> 
> 
> 



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