RE: double IHC/in situ
From: | "Connolly, Brett M" <brett_connolly@merck.com> |
Karen,
You may be interested in this article, J Histo Cyto 48(10): 1369-1375, 2000.
Brett
Brett M. Connolly, Ph.D.
Merck Research Laboratories
Department of Pharmacology
WP26A-3000
PO Box 4
West Point, PA 19486
Ph. 215-652-2501
FAX 215-652-2075
e-mail: brett_connolly@merck.com
> ----------
> From: Karen Larison[SMTP:larisonk@uoneuro.uoregon.edu]
> Sent: Thursday, January 11, 2001 3:02 PM
> To: HistoNet@Pathology.swmed.edu
> Subject: double IHC/in situ
>
> Hello Histonetters,
>
> Some of the researchers around here do in situ hybridization followed
> by IHC. However, the harsh conditions of the in situ hybridization
> techniques destroys many of the antigenic sites of interest. Does
> anyone out there use antigen retrieval to recover antigenicity
> following in situ techniques, or does anyone know of any references?
>
> Karen Larison
> University of Oregon
>
>
>
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