RE: cellulose and starch

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From:"Kellar, Eric" <kellarec@msx.upmc.edu> (by way of histonet)
To:histonet@histosearch.com
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Cellulose is PAS positive and will also stain with the direct azo dye Congo
red  (which is also known as a cellulose-substantive dye) demonstrating a
green birefringence.

Starch is also PAS positive, but is diastase labile (except for the
chemically altered starch used as a surgical glove powder). Starch will also
demonstrate a Maltese cross birefringence with polarization and cellulose
will not.



Eric C. Kellar
University of Pittsburgh Medical Center

	----------
	From:  Kathy Walters [SMTP:kwalters@emiris.iaf.uiowa.edu]
	Sent:  Friday, January 14, 2000 10:25 AM
	To:  HISTONET POST
	Subject:  cellulose and starch



	Dear esteemed colleagues,

	Here's what I think is a sticky one.  Anyone know how to stain and
	differentiate cellulose and starch?

	TIA,
	Kathy


	Kathy Walters                                                    / /
	Research Assistant III                                          / /\
	Center for Microscopy Research                                 / /\
\
	University of Iowa                                            /_/  \
\
	85 EMRB                                                      ____
((O))
	Iowa City, Iowa 52242                                        |  |  /
/
	                                                              ||  /
/
	email:  kwalters@emiris.iaf.uiowa.edu
-----------
	fax:  (319)335-8049
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	www:  http://www.uiowa.edu/~cemrf






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