RE: Which H&E?

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From:"Barry Rittman" <> (by way of histonet)
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I would agree with Wanda, phloxine is an excellent addition to the eosin.
Another option is to use a mixture of eosins, we used to use 4 parts of
eosin Y and 1 part of eosin B. We found that it was not so much a matter of
increasing the brilliance as increasing the range of eosin shades that were
present in the sections.

At 03:18 PM 01/11/2000 -0600, you wrote:
>We do people tissue, but what really made a difference in our H&E was
>"hooping up" our eosin with Phloxine.  If you are interested, I'll look up
>the formula for you it was quite simple to do.  (I'm up in the gross romm,
>my files are in the basement in EM) We also switched to Richard Allen Hemo,
>which was a vast improvement over the shall remain nameless yucky brand we
>were using.  let me know if I can help.
>Wanda Shotsberger
>Harris Methodist Hospital
>Fort Worth Texas
> ----------
>Subject: Which H&E?
>Date: Monday, January 10, 2000 5:03AM
>Hello Netters,
>I'm trying to brighten up my H&E stain.  It's been suggested that I switch
>Gill's #2 hematoxylin to Harris' hematoxylin.  I'm hesitant to do this,
>because I'm loathe to use mercuric oxide if it can be avoided.  Will this
>change make
>a big difference in the quality of my staining?  (I'm staining adult fish).
>Also, it's been recommended that I switch to an eosin containing a higher
>of alcohol.  This is no problem, but are there problems I might encounter by
>this switch?
>Karen in Oregon

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