RE: Protocol for H & E stain
|From:||Joan Yonchek <Jyonchek@rtitechnology.com>|
You should call the company and ask for a copy of the Customer Guideline
Manual which contains protocols for using their products. (800) 522-7270
In case you are in a hurry for the information I have included the
recommended staining procedure from the manual.
Station 1,2,3: Clear-Rite 3 or Xylene, 3 minutes each
Station 4,5,6: 100% flex or Dehydrant, 1 minute each
Station 7: 95% Flex or Dehydrant, 1 minute
Station 8: Rinse in running tap water, briefly
Station 9: Deionized or distilled water, rinse
Station 10: Hematoxylin 2, 3 - 5 minutes depending
upon desired contrast
Station 11: Running tap water, rinse off excess stain.
Station 12: Clarifier 2, 30 Sec - 1 Minute
Station 13: Rinse in running tap water, 30 Sec (agitate)
Station 14: Bluing Reagent, 1 minute
Station 15: Rinse in running tap water, 1 minute
Station 16: 95% Flex or Dehydrant, rinse
Station 17: Eosin-Y 0.5 - 2.5 minutes depending
Station 18,19,20 100% Flex or Dehydrant 1 minute each
Station 21,22,23 Clear-Rite or Xylene 1 minute each
Regeneration Technologies, Inc.
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