RE: OCT: What's in it? Is it good? Etc (Rather long)
<< Previous Message | Next Message >>
From: | "Tarpley, John" <jtarpley@amgen.com> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
> ----------
> From: J. A. Kiernan[SMTP:jkiernan@julian.uwo.ca]
> Sent: Tuesday, January 11, 2000 10:39 PM
> To: Linda Prentice
> Cc: histonet@pathology.swmed.edu
> Subject: Re: OCT: What's in it? Is it good? Etc (Rather long)
>
-snip snip-
> Unfortunately the identity of OCT isn't stated on the
> squeezy-bottle, and the same is true of other brand-name
> products that do the same job.
John,
My squeezy-bottles of O.C.T. state the following: "Contents: 10.24% w/w
polyvinyl alcohol; 4.26% w/w polyethylene glycol; 86.50% w/w nonreactive
ingredients."
> A reasonable guess might
> be that they contain water and polyethylene glycols of
> quite high molecular weight. Gelatin (about 2% in water)
> seems to work about as well. Some HistoNet discussion in
> the past indicated that the initials stood for Optimum
> Cutting Temperature. This makes no sense in the context
> of cryostat sectioning, but it may do so if you are using
> an old-fashioned open-air freezing microtome (as do many
> of us who work with thickish sections of CNS, muscle or
> skin for research purposes).
The name O.C.T. may make no sense in your context, but the squeezy-bottle
also states, "Freeze to optimal cutting temperature." I've also understood
the name to be an acronym for Optimal Cutting Temperature, but perhaps some
former Tissue-Tek person or one of the Sakura people could enlighten us
further. I think we all know that product brand names don't always follow a
totally logical convention. I've tried both water and O.C.T. for frozen
cryostat sections and definitely prefer O.C.T.
John Tarpley 15-2-B
Associate Scientist
Specialist Image Analysis & Immunohistochemistry
Amgen Inc
One Amgen Center Drive
Thousand Oaks, CA 91320
<< Previous Message | Next Message >>