Hi Geoff

	I recall using scarba red many years ago when I was a trainee in the
pathology 
	department at Edinburgh university medical school. I think that it
was particularly good 
	as a nuclear counterstain for reticulin impregnations, where the
nuclear staining can be
	 weak. I no longer have the recipe so I'd be grateful if you could
send me a copy.

	TIA Charles


Charles McKinney
Chief Biomedical Scientist
Histopathology Deptartment
Frenchay Hospital
Bristol



> From: 	Geoff McAuliffe[SMTP:mcauliff@UMDNJ.EDU]
> Sent: 	05 January 2001 16:26
> To: 	Andrea Grantham
> Cc: 	histonet@pathology.swmed.edu
> Subject: 	Re: Iron stain counterstain
> 
> Andrea Grantham wrote:
> 
> > Histonetters -
> > I was wondering if there was another stain (other than Nuclear Fast Red)
> > that could be used as a counterstain for the iron stains?
> 
>     I like "Scarba Red", an acidified neutral red stain. It is a bit of a
> pain
> to make and does require some differentiation to get a pure nuclear stain,
> but
> it is a nice, bright red (with a bit of orange). I will send the recipe if
> you
> like. You will need analine and phenol to make it, as well as the neutral
> red.
> 
> >
> > My next question is - what can be done to prevent the white precipitate
> > from adhering to the slides that have been in Nuclear Fast Red
> > (Kernechtrot)Solution? I have had this problem with several batches of
> the
> > stain - not just in this lab with these chemicals. I am sure that I am
> > making up the solution correctly and just to be certain I had another
> tech
> > make it up using the "recipe" from the green AFIP book (page 179). Still
> > the same problem.
> 
> I have never had this problem.
> 
> Geoff
> --
> **********************************************
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583; fax: -4029
> mcauliff@umdnj.edu
> **********************************************
> 
> 
>