RE: Iron stain counterstain

From:dizefamily <dizefamily@zyan.net>

i found that if you don't rinse in h2o before going to alcohol you get that
precipitate. Rinse in h20 then dehydrate.
Lynn
Piedmont Hospital
Atlanta ,gA

-----Original Message-----
From: Andrea Grantham [mailto:algranth@u.arizona.edu]
Sent: Friday, January 05, 2001 10:44 AM
To: histonet@pathology.swmed.edu
Subject: Iron stain counterstain


Histonetters -
I was wondering if there was another stain (other than Nuclear Fast Red)
that could be used as a counterstain for the iron stains?
My next question is - what can be done to prevent the white precipitate
from adhering to the slides that have been in Nuclear Fast Red
(Kernechtrot)Solution? I have had this problem with several batches of the
stain - not just in this lab with these chemicals. I am sure that I am
making up the solution correctly and just to be certain I had another tech
make it up using the "recipe" from the green AFIP book (page 179). Still
the same problem.
Any advice/help would be greatly appreciated since this is driving me nuts!
Thanks!
Andi
.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
: (office:  AHSC 4212)          P.O. Box 245044                     :
: (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
: (FAX:  520-626-2097)          (email:  algranth@u.arizona.edu)       :
:...................................................................:
           http://www.cba.arizona.edu/histology-lab.html





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