RE: Cryo-protectants

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From:"Kellar, Eric" <> (by way of histonet)
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Tokuyasu has shown that sucrose solutions that are over 1.6 M are far
superior and can be frozen by immersion in liquid nitrogen without ice
crystal damage. Sucrose will easily penetrate fixed tissues and should not
be used as a cryo-protectant for unfixed material. Glucose, glycerol,
ethylene glycol, 2-methyl-2,4-pentadiol (MPD), dimethyl sulfoxide (DMSO),
PEG 400, erythritol, a methoxylated version of propylene glycol and
(2R,3R)-(-)-butan 2,3-diol have also been used successfully as
Results of course differ depending upon the application.

Eric C. Kellar
University of Pittsburgh Medical Center

> ----------
> From: 	Paul Popper[]
> Sent: 	Tuesday, January 04, 2000 11:24 AM
> To: 	Histonet
> Subject: 	(no subject)
> Hi, does glucose work as a cryoprotectant or it has to be sucrose?
> Thanks,
> Paul Popper

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