RE: Cork discs.

From:"Sebree Linda A." <la.sebree@hosp.wisc.edu>

Regarding the cork disc issue; I used these when doing research on rabbit
myocardium.  They are much cheaper (I bought a bag of corks and had my
husband slice them up on his band saw) than metal object holders and take up
less storage space in the freezer.  Also, there is much less chance of
thawing your tissue when removing it from the metal object holder if you
have the insulating cork there.  I simply applied gum taraganth (sp?), or
you could use something like Instrumedics' Cryo-Gel, to the slice of cork,
positioned my tissue on it and froze the whole works in isopentane cooled by
liquid nitrogen.  When ready to section, you just mount the bottom of the
cork onto a metal object disc.

This worked pretty slick for me.

Linda A. Sebree, HT
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory, D4/218
600 Highland Ave.
Madison, WI 53792-2472
(608)265-6596
FAX: (608)263-1568
<la.sebree@hosp.wisc.edu>


-----Original Message-----
From: Corazon D. Bucana [mailto:bucana@audumla.mdacc.tmc.edu]
Sent: Monday, January 08, 2001 7:14 AM
To: Mark Hammond
Cc: histonet@pathology.swmed.edu
Subject: Re: Cork discs.


As a latecomer to frozen sectioning etc.  I was wondering about the
rationale
for the cork discs.  I have received frozen samples from Europe for a
collaborative study and I wondered about the rationale for mounting samples
on
cork discs.   It seems to me that cork doesnt conduct heat very well as
opposed
to metal.  Does this affect the preservation of the samples?  
Thanks you,

Cora

 At 01:07 PM 1/8/01 +0000, you wrote: 
>
> Ian, 
>
> RA Lamb for cork discs. 
>
> Mark 
>
> ************************** 
> Mark Hammond 
> Laboratory Manager 
> Pathology Central Services 
> University of Cambridge 
> 01223 333905 
> mjh80@cam.ac.uk 
> ************************** 
>
> "Dr. Ian Montgomery." wrote: 
>>
>>         A question for UK Histologists. 
>>         After many years I'm running out of cork discs for cryostat
specimens
>> and unfortunately I can't remember who supplied them. Please, anyone
using
>> cork discs, where did you buy them. 
>> Ian. 
>>   
>>
>> Dr. Ian Montgomery, 
>> West Medical Building, 
>> University of Glasgow, 
>> Glasgow, 
>> G12 8QQ. 
>> Tel: 0141 339 8855.  Extn:6602. 
>> Fax: 0141 330 2923 
>> e-mail: ian.montgomery@bio.gla.ac.uk
>
>
> -- 
>   
>  



Corazon D. Bucana, Ph.D.
Dept. Cancer Biology
UT M.D.Anderson Cancer Center
1515 Holcombe Blvd, Box 173
Houston, Texas 77030
Phone: 713-792-8106
FAX 713-792-8747



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