I just read Freida Carson's notes on this method - she says that sections
that are too thick show yellow birefringence (Bancroft and Stevens)
Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta


	-----Original Message-----
	From:	Jennings-Siena, Debbie
[SMTP:ds.jennings-siena@baylordallas.edu]
	Sent:	Thursday, January 13, 2000 10:41 AM
	To:	'histonet@pathology.swmed.edu'
	Subject:	Congo Red Stain problems

	  Hi everyone,
	I would like to ask if anyone knows why amyloid on Congo Red stain
would
	birefringe as a orange-yellow color instead of apple green?  We are
using
	the same procedure that we have used for years.  Our procedure is a
Puchtler
	Modification.  If anyone has any ideas, I would appreciate hearing
from you.
	Thanks in advance.

	Debbie J. Siena, HT(ASCP)QIHC
	Histopathology Manager
	Baylor University Medical Center
	Dallas, TX 75246
	214-820-2465