We are using a polyclonal CD3 prediluted from Ventana/Cell Marque, stained
on Ventana ES and NexES.  No HIER.  Protease 1 for 4 min.   Pimary
incubation for 12 min.

> -----Original Message-----
> From:	Richard Cartun [SMTP:Rcartun@harthosp.org]
> Sent:	Thursday, January 06, 2000 10:35 AM
> To:	Histonet@pathology.swmed.edu
> Subject:	CD3
>
> I am interested in hearing what Histoneters are using for the detection of
> CD3+ cells in paraffin sections (pAb vs. mAb, digestion vs. HIER, etc.).
> Thanks.
>
> R. Cartun