RB, MDM2, p16INK4
|From:||Leo Levesque <LLevesque@exchange.hsc.mb.ca>|
We have tried to get the following antibodies working for us but have not
had any success.
Mouse Retinoblastoma Protein (RB) -from PharMingen Int. Cat # 14001A
MDM2 -from Santa Cruz Biotechnology, Inc. Cat # SC-965
p16INK4 -from ParMingen Int Cat # 13251A
We have followed suggestions on the spec sheets, tried these with and
without antigen retrieval. We have also tried using high sensitivity
detection systems but without success.
I would like to hear from anyone having experience with these antibodies.
If the antibody did work, please send a protocol.
Also, I am interested in knowing what other companies supply these
antibodies and if anyone has implemented these techniques.
Health Sciences Centre
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