Iron stain counterstain

From:Andrea Grantham <algranth@u.arizona.edu>

Histonetters -
I was wondering if there was another stain (other than Nuclear Fast Red) 
that could be used as a counterstain for the iron stains?
My next question is - what can be done to prevent the white precipitate 
from adhering to the slides that have been in Nuclear Fast Red 
(Kernechtrot)Solution? I have had this problem with several batches of the 
stain - not just in this lab with these chemicals. I am sure that I am 
making up the solution correctly and just to be certain I had another tech 
make it up using the "recipe" from the green AFIP book (page 179). Still 
the same problem.
Any advice/help would be greatly appreciated since this is driving me nuts!
Thanks!
Andi
.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
: (office:  AHSC 4212)          P.O. Box 245044                     :
: (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
: (FAX:  520-626-2097)          (email:  algranth@u.arizona.edu)       :
:...................................................................:
           http://www.cba.arizona.edu/histology-lab.html




<< Previous Message | Next Message >>