Cryopreservation solution for tissue slices?

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From:Bret Morrow <> (by way of histonet)
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I am stuck with a freezer which sneaks down to -30 oC on weekends when I
am not hear to yell at it.  The problem is that I have loads of 50 um
brain tissue saved in cryopreservation solutions which only protect down
to about -20 to -25 oC.  To avoid damage from ice crystals, I would like
to use a cryo solution which could go a bit lower.  Does anyone have a
receipe for such a solution.  The one we are currently using is as

Sucrose                                    150 g
Ethylene Glycol                        150 ml
Phosphate buffer (0.1 M)         250 ml
make upto 500 ml with water

Any suggestions would be helpful.

Bret A. Morrow, Ph.D.
Associate research scientist
Associate clinical professor
Yale University School of Medicine
New Haven, CT 06520-8066

voice 203.785.4515
fax  203.785.7670

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