Cryopreservation solution for tissue slices?
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From: | Bret Morrow <bret.morrow@yale.edu> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Greetings,
I am stuck with a freezer which sneaks down to -30 oC on weekends when I
am not hear to yell at it. The problem is that I have loads of 50 um
brain tissue saved in cryopreservation solutions which only protect down
to about -20 to -25 oC. To avoid damage from ice crystals, I would like
to use a cryo solution which could go a bit lower. Does anyone have a
receipe for such a solution. The one we are currently using is as
follows:
Sucrose 150 g
Ethylene Glycol 150 ml
Phosphate buffer (0.1 M) 250 ml
make upto 500 ml with water
Any suggestions would be helpful.
TIA
____________________________________________
Bret A. Morrow, Ph.D.
Associate research scientist
Associate clinical professor
Yale University School of Medicine
New Haven, CT 06520-8066
voice 203.785.4515
fax 203.785.7670
email bret.morrow@yale.edu
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