Background in CD3 stains
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| From: | "Nicola Falconer" <njfja@njfja.screaming.net> (by way of histonet) |
| To: | histonet@histosearch.com |
| Reply-To: | |
| Content-Type: | text/plain; charset="us-ascii" |
Dear Dana
Sorry if I am stating the obvious, but the most common cause of background
in immunos is the antibody being too strong. Try diluting out a bit more
e.g. if using 1/100 try 1/200 to 1/1000 or onwards and see what happens.
Good luck
John Auld
Immunocytochemistry
Royal Free Hospital
London
U.K.
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