Anti-H. Pylori Question
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From: | "Sandy Julsing" <sandy.julsing@thedacare.org> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Hello Histonetters,
I have two questions re: anti H.Pylori.
~We are using DAKO's antibody at 1:400 15 minutes
~10 min AR in steamer and citrate buffer pH 5.8
~LSAB+ 15 min, 15 min, 5 min
My questions are...
1-How far out have others diluted the anti-H Pylori and using which antigen
retrieval and detection kit?
2-Do you do a rapid Giemsa or Diff-Quik first and use the anti H.Pylori
only if it is negative?
Thanks a bunch - Sandosis
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