Anti-H. Pylori Question

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From:"Sandy Julsing" <> (by way of histonet)
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Hello Histonetters,
   I have two questions re: anti H.Pylori.
~We are using DAKO's antibody at 1:400 15 minutes
~10 min AR in steamer and citrate buffer pH 5.8
~LSAB+ 15 min, 15 min, 5 min

My questions are...

1-How far out have others diluted the anti-H Pylori and using which antigen
retrieval and detection kit?

2-Do you do a rapid Giemsa or Diff-Quik first and use  the anti H.Pylori
only if it is negative?

Thanks a bunch - Sandosis

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