AW: Alcec Blue & mast cell staining
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From: | "Nader, Alexander" <alexander.nader@wgkk.sozvers.at> (by way of histonet) |
To: | histonet@histosearch.com |
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unfortunately I didn't eceive the original mail from Jim Manavis, so I would
like to comment on Alcian blue staining for mast cells.
There's a not very well known method published by Csaba 1969 (Acta
biologicae academicae scientiarum hungaricae, 20, 205) on a double staining
technique for mast-cells using alcian blue and safranin. It can be found
also in Bancroft & Stevens book "Theory and Practice of Histological
Techniques". It is a pretty nice stain, showing mast-cells in two different
colours (depending on their granula) and we use it in combination with
immunohistochemistry for Tryptase (DAKO). I often wonder about the quantity
of mast-cells in BM-trephines shown by these two methods in comparison with
the routine Giemsa stain.
Dr. Alexander Nader
Path. Institut Hanuschkrankenhaus
A 1140 Wien, Oesterreich
Alexander.Nader@wgkk.sozvers.at <mailto:Alexander.Nader@wgkk.sozvers.at>
privat: Alexander.Nader@univie.ac.at
<mailto:Alexander.Nader@univie.ac.at>
> -----Ursprüngliche Nachricht-----
> Von: J. A. Kiernan [mailto:jkiernan@julian.uwo.ca]
> Gesendet am: Mittwoch, 05. Jänner 2000 18:08
> An: Jim Manavis
> Cc: Histonet
> Betreff: Re: Alcec Blue & mast cell staining
>
> On Wed, 5 Jan 2000, Jim Manavis wrote:
>
> > I am after some help looking for a discontinued product
> from Sigma-Aldridge
> > called Alcec Blue which is a histochemical stain being used for
> > discrimination of mast cells.
>
> Alcec blue was (? still is) a substitute for alcian blue.
> The name is a trademark of the Hoechst company for a dye
> synthesized by JE Scott (J. Microsc. 119:373, 1980). A short
> account is given by F. Green in the Sigma-Aldrich Handbook of
> Stains, Dyes and Indicators (1991), p.69-70.
>
> You should have no difficulty obtaining alcian blue. Sigma-Aldrich
> also sell a "pyridine variant" of alcian blue, which may be more
> stable than the original dye. For fairly selective staining of
> mast cells, use a solution of the dye at pH 1.0. In fact, any
> basic (cationic) dye used at pH 1 will stain mast cells and other
> objects that contain sulphated carbohydrates. The advantage of
> alcian blue is that it is not removed by subsequently applied
> other stains or solvents.
>
> John A. Kiernan,
> Department of Anatomy & Cell Biology,
> The University of Western Ontario,
> LONDON, Canada N6A 5C1
>
>
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