testis fixation/ISH/Bouins
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From: | Gayle Callis <uvsgc@msu.oscs.montana.edu> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
To our colleague "Down Under" and others interested in ISH technics, and
other DNA/RNA molecular biology methods (RT-PCR), Bouins is not considered
an good fixative for ISH/RT-PCR. Acid containing fixatives cause
DNA destruction. Formalin containing fixatives, NBF, zinc formalin, formol
saline, paraformaldehyde are fixatives of choice. Also avoid alcohol/acetone.
Zenkers, B-5 and 2% gluteraldehyde (crosslinks too much!)
Rationale is to use crosslinking fiative to keep DNA in place.
I learned this from two ladies, Christine Bromely and Mary Maher-Sturm -
experts at doing this kind of work, in their outstanding workshop.
Gayle Callis
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