antbody storage

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I agree with you completely.  To the best of my knowledge no paper has
written.   It would be difficult to do without the cooperation of the
manufacturers as we don't know how the antibodies are stored, or how
long, prior to
packaging and shipment.  I would hazard a guess that once the antibodies
aliquoted they are probably stored at -80 untill shipment ( I could be
happens all the time).

-- Begin original message --

> From: "J. A. Kiernan" <>
> Date: Mon, 22 Feb 1999 12:13:15 -0500 (EST)
> Subject: Re: Antibody storage (temperature, time)
> To:
> Cc: Ian Montgomery <>,
>   You'd think the immunologists would have sorted out optimal
>   storage conditions years ago. I have talked to a few local
>   ones, and they  work by "common sense" and hearsay:
>   avoid repeated freezing/thawing; use -80; -20 is OK;
>   +4 and throw out in 6 months; etc. No consistency at all.
>   A general suspicion exists of suppliers' expiration dates,
>   which might be contrived to make us buy more often.
>   Has anyone seen a published comparative study, in a
>   regular peer-reviewed journal, of the effects of
>   different storage conditions on different kinds
>   of antibodies?
>                    John Kiernan, London, Canada.
> On Mon, 22 Feb 1999 wrote:
> > I have been using antibodies that were made for us ~12 years ago
which were
> > aliquated and stored in one of our -80 freezes and they still work
> > perfectly.  Don't know about storing them at -196, I would doubt
that it
> > would hurt them but I don't think that it would extend their
> >
> > I do know that when these same antibodies were stored at -20 (some
were put
> > in the -20 by a predecessor) they started to lose potency after
about 3
> > years.  That was when I 'discovered' the ones stored in the -80.

best regards,
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
Chapel Hill, NC 27599
office 919-966-6343
   Lab 919-966-6140
   Fax 919-966-6123

**Suppose you were an idiot... And suppose you were a member of Congress
But I repeat myself.-Mark Twain**

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