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From:Jim Elsam <> (by way of histonet)
To:histonet <>
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Rita Angel writes:
        Our lab has been having a problem lately with our Masson's
Trichrome.... Our muscle has been staining purple instead of red.... and
we were purchasing the Gomori's stain pre-made.


The red dyes in trichromes are to some extent interchangeable. Here in
the UK, where Masson's is favourite, the older methods used a mixture of
dyes whereas the new ones use straight acid fuchsin. This results in a
much more purple coloration of muscle.

My guess would be that your supplier has changed the brew and is now
using acid fuchsin instead of chromotrope 2R.

Good luck!
Jim Elsam
HTEQA Services

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