Judi,
Formol Sucrose.
0.2M. phosphaste buffer (pH7.4).  =  90ml.
sucrose.                                             =  7.5g.
40% formalin.                                   =  10ml.

	Try the following method, it works a treat.
	If you don't have the journal let me know and I'll e-mail the
method. It works on fresh, fixed, paraffin processed and resin sections.
Tetrazolium method for non-specific Alkaline Phosphatase.
McGadey, J.  1970.  Histochemie.  23.  180-184.
Ian.



>Date: Mon, 22 Feb 1999 17:20:19 -0800
>From: "Ford, Judi {PATH~Palo Alto}" <JUDI.FORD@roche.com>
>Subject: Sucrose buffered formalin
>To: "Histonet (E-mail)" <histonet@pathology.swmed.edu>
>MIME-version: 1.0
>
>Hello Everyone:
>I have a pathologist who wants an indoxyl-tetrazolium method for alkaline
>phosphatase done on some cross-sections of fresh frozen muscle tissue.  The
>problem I have is that the method I'm using calls for a post-fix in sucrose
>buffered formalin and no recipe was described for the buffer in the paper.
>Does anyone have a recipe for the buffered formalin, or can suggest where to
>look?
>
>Any and all help is greatly appreciated.
>
>Judi Ford
>Roche Bioscience
>Palo Alto, CA
>
>Judi.Ford@roche.com
>

Dr. Ian Montgomery,
West Medical Building,
University of Glasgow,
Glasgow,
G12 8QQ,
Scotland.
Tel: 0141 339 8855 Extn. 6602.
Fax: 0141 330 4100.
e-mail: ian.montgomery@bio.gla.ac.uk