Ann,
You could try either monoclonal antibody from Dako (M0776, necessary to use
microwave cooking in citrate buffer, dil.1:50-1:100, Strept/HRP) or
monoclonal from BioGenex (clone Snp88, no pretreatment, dil. 1:200-1:300,
Strept/HRP). I tried them on animal tissues but you should not have any
problem with human material.

Grazyna





ANN MARUSKA <AMARUSK1@FAIRVIEW.ORG> on 11.02.99 22:09:57

To:   HistoNet@Pathology.swmed.edu
cc:    (bcc: Grazyna Wieczorek/PH/Novartis)
Subject:  synapto




Hi Histonetters!
 My IHC pathologist has been complaining that the Synaptophysin we use is
not specific enough...it's staining more things than what she wants. I am
currently using Dako's #A010 at a 1:75 titer on the NexES with protease #1.
Has anyone else experienced this complaint?  What are you folks out there
using?  This inquiring mind wants to know!
 Thanks in advance.
Ann Maruska
AMARUSK1@fairview.org
Fairview-University Med Ctr
Mpls. MN 55454