Re: retic stain

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From:Ken Turner <ken.turner@stonebow.otago.ac.nz> (by way of histonet)
To:histonet <histonet@magicnet.net>
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I have successfully used Long's method for reticulin for many years. It has
the added advantage that the ammoniacal silver solution is stable for some
months - depending on the frequency of use and how well it is looked after.
You can find the protocol in: Stain Technology, Vol 23, No.2 April 1948 pp
69-75.

I hope this is of some use.

Regards

Ken

>Can anyone recommend the best method for staining reticular fibers?
>I'm using Gomori retic and I've been getting inconsistent results,
>either too light or too dark.  I've changed times, solutions, etc.
>Help!  Thanks so much!
>
>M.


Kenneth W Turner
Senior Teaching Fellow
Unit Manager / Pathology Histology Unit
Department of Pathology
Otago Medical School
P.O.Box 913
Dunedin
New Zealand

Tel. 03 4797135  International  0064 3 4797135
Fax. 03 4797136  International  0064 3 4797136




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