Re: omiting the primary Ab for IHC control,

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From:Katri Tuomala <> (by way of histonet)
To:histonet <>
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Bruce A Rasmussen wrote:
> Is it general procedure to omit the primary Ab with a slide or two every
> time IHC is run or just a couple of times when piloting the Abs for
> control?  Thanks in advance. The know how of this list serve really amazes
> me.
> Bruce
> --------------------------------------
> Bruce Rasmussen
> Predoc Fellow in Experimental Neuropsychology
> The Krasnow Institute for Advanced Study
> George Mason University
> Mail Stop 2A1
> Fairfax, VA 22030-4444
> Office:703-993-4358
> Lab:703-993-4369
> Home:703-765-4570
> Fax:703-993-4325
> ---------------------------------------

Hi Bruce,
We run a "negative" control slide from each case with each method:
meaning, that if there are both polyclonal and monoclonal antibodies to
be used with different detection systems, a "neg" control is run through
both. In addition: if antigen retrieval is required for some and not the
others, a "neg" control is run through both. So you may end up with four
"neg" control slides in one case. Some may think this is an overkill,
but I feel more in control by detecting any possible non-specific
staining by detection systems, particularly when antigen retrieval is
being used.

Katri Tuomala
Anatomic Pathology
St.Joseph's Hospital
Hamilton, Ontario, Canada

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