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>Date: Fri, 26 Feb 1999 18:49:39 -0500 (EST)
>From: Arlene N Taylor <cloud39@juno.com>
>Subject: hematoxylin & eosin  frozen section staining
>To: histonet@pathology.swmed.edu
>Message-id: <19990226.195519.7447.0.cloud39@juno.com>
>
>Hi,
>I'm collecting  procedures for hematoxylin and eosin  of staining
frozen
>sections.
>Thanks in advance.
We fixed our frozen sections in 10% neutral buffered formalin for 1
minute, followed by a tap and distilled rinse, stain in Mayers
hematoxylin for 2 minutes, blued in 1% ammonia water, rinsed in water,
dipped in 95% alcohol, stained 3 dips in alcoholic eosin, dehydrated in
2 changes of 95% alcohol, 2 changes of 100% alcohol, and 3 changes of
xylene, 10-15 dips each.
>
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