I have an article by Braak and Braak (Brain Pathology 1:213-216. 1991)
which demonstrates amyloid deposits and neurofibrillary changes using a
combination Campbell/Gallyas stain. I wrote up a one page protocol which I
could fax to you directly if you wish. I did the stain a few times, and
recall the slides developing in 3-5 minutes, although the article states
that development can take from 5-30 minutes, depending on the tissue. Is
your tissue old, or are you unsure about how it was processed. This could
affect development times. Reply directly to me if you want me to fax you my
protocol.
Joyce Kotzuk, Univ. of New Mexico


>>> Rod Slyter <rlslyter@hsc.vcu.edu> 02/18/99 05:44AM >>>
Histonetters:
I am in need of some assistance on a neuro special stain: I have
procedure for a gallyas stain for nuerofibullart tangles and other
finding for neurologic disorders. This stain was given to me by my
neuropath. It is from an journal article. The procedure uses an alkaline
iodide silver solution which is developed by 3 solution combination
silver.ammonium/tunstosilicic acid solution. The procedure says the
slides should develop in 5-10 minutes but mine seem to take around 35
minutes. Does anyone know where I can get the actual procedure instead
of a piecemeal article.

Thanks Rod Slyter