Re: antigen retrival

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From:vandeplas@aurion.nl (Peter van de Plas) (by way of histonet)
To:histonet <histonet@magicnet.net>
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>Cindy wrote:
>    I am going to be using microwave antigen retrival for a couple of
>antibodies that I have not previously stained with. I was wondering
>if anyone had any recipes for Citrate Buffers or any other
>buffer solutions used for this purpose?
>
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Dear Cindy,
The optimal AR protocol differs from primary  to primary.
The review article by Shan-Rong Shi might be of help.
Shi et al (1997) J. Histochem. Cytochem. 45, 327-343
Peter

========================================
Peter van de Plas
AURION
Costerweg 5
6702 AA Wageningen
The Netherlands
phone: (31)-317-497676
fax: (31)-317-415955
http://www.aurion.nl




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