Re: antigen retrival

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>Cindy wrote:
>    I am going to be using microwave antigen retrival for a couple of
>antibodies that I have not previously stained with. I was wondering
>if anyone had any recipes for Citrate Buffers or any other
>buffer solutions used for this purpose?
Dear Cindy,
The optimal AR protocol differs from primary  to primary.
The review article by Shan-Rong Shi might be of help.
Shi et al (1997) J. Histochem. Cytochem. 45, 327-343

Peter van de Plas
Costerweg 5
6702 AA Wageningen
The Netherlands
phone: (31)-317-497676
fax: (31)-317-415955

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