Re: anti-SMA

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From:"M. Brown" <marianb@u.washington.edu> (by way of histonet)
To:histonet <histonet@magicnet.net>
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In the past I did some research for an angiogenisis study on prostate
tissue. I I will have to look up my notes on methacrylate material (If I
can find them)but I had also used UEA(Ulex),CD34 and CD31. You can call
David
Tacha at Biocare Medical 1-800-799-9499. Another contact would be Dr. Jay
Beckstead (I use to work for him and we did lots of ICC on methacrylate
material. Jay was probably the first reference known for ICC staining on
methacrylate material. He is now in Medford ORE. 541-608-4191  I think his
e-mail is jbeckstead@asante.org
Marianne Brown




On Tue, 16 Feb 1999, John Bliss wrote:

> Dear Jan Willen de Groot:
>
> Concerning IHC staining of vessel walls embedded in methacrylate: I would
> recommend immunogold labeling of methacrylate-embedded tissues (see Tacha,
> D. et al. J Histotech 16(1):13-17 (1993). Zymed makes HistoGold(tm) kits
> which are ideal for this use. Also if you are having trouble with the SMA
> antibody, you might consider anti-CD34 which reacts with hemopoietic stem
> cells and small-vessel endothelial cells.
>
> Regards,
> --
> Sincerely,
>
> John Bliss
> Zymed Laboratories, Inc
> 458 Carlton Court
> So. San Francisco, CA 94080
> Tel: 800-871-4494
> FAX: 650-871-4499
> E-mail: histo@zymed.com
> Website: www.zymed.com
>
> --
> Jan Willem de Groot wrote:
>
> > Hello Histonetters,
> >
> > We are interested in immunohistochemical staining of human vessel walls.
> > For this reason we have some problems with anti-SMA in tissues that are
> > embedded in Technovitt 8100. Is somebody familiar with anti-SMA
> > immunohistochemical staining in tissues that are embedded in Technovitt
> > 8100 (methacrylate)?
> >
> > I thank you in advanced,
> >
> > Greetings,
> >
> > Jan Willem de Groot
> >
> > J.W. de Groot
> > Utrecht University
> > Dept. of Functional Anatomy
> > P.O. Box 80039
> > 3508 TA Utrecht, the Netherlands
> > tel.+31 30 2538323 fax +31 30 2539030
> > e-mail j.degroot@fa.ruu.nl
> >                                   ###
> >                                  (@ @)
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> >                   |       Jan Willem de Groot         |
> >                   |          Veldoven 25              |
> >                   |    3961 ET Wijk bij Duurstede     |
> >                   |         0343 - 574786             |
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>
>
>
>
> --
>
>
>
>




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