Re: Toxoplasma

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From:"Bill Sinai (Anatomical Pathology)" <Bills@icpmr.wsahs.nsw.gov.au> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Date:          Thu, 18 Feb 1999 15:51:55 +1000
From:          Laurence Reilly <laurie.reilly@jcu.edu.au>
Subject:       Toxoplasma
To:            HistoNet@Pathology.swmed.edu

Laurie,

Try dilute (1:40 in distilled water) Giemsa for 30 min at 60
degrees,  then rinse in absolute ethanol, briefly xylene and mount.
By varying the time in dilute giemsa you can make the staining
lighter or heavier.

Hope this works for you.
*********************************************************************
 Hi everyone, Can anyone help me with a stain to
demonstrate Toxoplasma organisms in tissue sections? Thanks in
advance.                     Regards,  Laurie.

Mr.Laurie Reilly                               Phone   07 4781 4468
Histopathology                                 Facsimile   07 4781 5558
Physiology and Pharmacology                    Email   laurie.reilly@jcu.edu.au
Aust.Institute Tropical Vet & Animal Sc.
James Cook University.
Townsville.  4811     Australia.


Bill Sinai
Department Manager
Tissue Pathology
ICPMR Westmead Hospital
WESTMEAD NSW AUSTRALIA
Phone 61+2+9845 7774  Fax 61+2+9687 2330




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