Re: Sucrose buffered formalin

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From:Joachim Siegmund <jsiegmu@ibm.net> (by way of histonet)
To:histonet <histonet@magicnet.net>
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"Ford, Judi {PATH~Palo Alto}" wrote:

> Hello Everyone:
> I have a pathologist who wants an indoxyl-tetrazolium method for alkaline
> phosphatase done on some cross-sections of fresh frozen muscle tissue.  The
> problem I have is that the method I'm using calls for a post-fix in sucrose
> buffered formalin and no recipe was described for the buffer in the paper.
> Does anyone have a recipe for the buffered formalin, or can suggest where to
> look?
>
> Any and all help is greatly appreciated.
>
> Judi Ford
> Roche Bioscience
> Palo Alto, CA
>
> Judi.Ford@roche.com

Hi Judi,

I am sure you can use normal NBF ( 30 min ) too. Especially in  the case of
post-fixation there should be no negative effects.
( see Romeis  1127 )

Hope it's a help

Joachim


Joachim Siegmund
BTA
Hamburg,Germany




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