Re: Jenkin's solution
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From: | RUSS ALLISON <Allison@cardiff.ac.uk> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
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Content-Type: | text/plain; charset="us-ascii" |
Jenkin's Fluid:
Concentrated hydrochloric acid 4ml
Glacial acetic acid 3ml
Distilled water 10ml
Absolute alcohol (i.e. 100%) 73ml
Chloroform 10ml
Reasonably quick, haematoxylin staining quite well preserved.
For subsequent processing, it is permissible, indeed, encouraged,
that the tissue go straight into absolute alcohol, , then chloroform
and subsequent paraffin wax. (each for appropriate times, of course
and dependent upon block size, etc.)
Many years ago (wish I was too young to be able to say that!), when
working in general pathology, I used it fairly extensively with
good results, but probably not obviously better for routine work
than the 10-12% aqueous formic acid we use now.
Ref. Jenkins, CE. J Pathology & Bacteriology, 1921;24:166.
Amusingly, according to Lillie, the time of decalcification presumes
an ambient temperature of 17-20 degrees C in Glasgow!!
Russ Allison, Wales
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