Re: IHC titer
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From: | Phyllis Davie <pdavie@phenopath.com> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="iso-8859-1" |
I'll address the question that I haven't seen answered yet. I do keep
titer records, but I don't retain titer slides. (I'd be drowning in them
by now!) I do, however, keep 1 or 2 examples of "good" staining on each
antibody. I have a separate alphabetical file for these, and I have found
it to be invaluable for times when I feel that the antibody has "drifted",
or for rarely used antibodies, or when I have visitors who want to see what
a specific antibody should look like, or when my boss wants to take a
picture of "just one good example of ......(whatever).
>Hi Histoinans !
> I would like some help with titering my immunohistochemical primary
>antibodies, please.
> How many dilutions and in what ranges. What does an economical pipette for
>this procedure cost? Do you use titer records & retain titer slides? Do you
>adjust antibodies on special request from the Pathologist to demonstrate
>specifically “hot” markers?
> I’ve told my Pathologists that we need to invest in the pipette for proper
>titering procedure. I keep records of the dilutions we arrive at and make the
>Pathologist sign off on each lot of primary antibody.
> Thank You for any help
> Respectfully
> Verrucous
>
>
>
Phyllis Davie
Clinical Laboratory Supervisor
PhenoPath Laboratories
Seattle, WA
pdavie@phenopath.com
(206)374-9009 phone
(206)374-9009 fax
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