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From:Edward Henry <> (by way of histonet)
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Please unsubscribe from the HistoNet.  Thank You

---HistoNet Server <> wrote:
> ----------------------------------------------------------------------
> Date: 24 Feb 1999 02:30:13 -0600
> From: Keith Miller <>
> Subject: Latest on Cyclin D1 immunostaining
> Dear Cyclin D1 sufferers,
> For several years we employed 1mM EDTA pH8.0 retrieval for cyclin
D1, but
> have recently changed to the Dako pH9.9 Target Retrieval Solution.
> Microwave Oven heat in 600ml of this solution, starting from cold,
for 25
> minutes,  and flush out with cold running water at the end of the
> cycle. Alternatively, the fluid can be saved and re-used, by
placing the
> hot container in cold running water for 10 minutes and then promptly
> transferring the slides into the cold water.
> Incubate in Dako clone DCS-6 at 1/100 for one hour and label with
> avidin-biotin px etc. [DCS-6 is also available from many other
> The pH9.9 fluid is much more efficient than pH8.0 EDTA and has made
> demonstration of Cyclin D1 in mantle cell lymphoma as reliable as
> cytokeratin antibodies for labelling epithelium.
> However, I would not recommend using this high pH retrieval solution
in a
> pressure cooker as sections can sometimes detach.
> I hope this helps.
> Unfortunately, I no longer subscribe to the Histonet on a regular
basis, so
> if further details are required, please feel to contact me directly.
> Keith Miller
> Royal Free & University College London Medical School
> ----------------------------------------------------------------------
> Date: 24 Feb 1999 02:56:00 -0600
> From: "Marshall, Sharon, Mrs" <>
> Subject: Re: normal tissues ? CHTN
> > Date:          Fri, 19 Feb 1999 10:14:21 +0000
> > From:          Alexander Brands <alexander.brands@uni-tuebingen.d
> > Subject:       Re: normal tissues ? CHTN
> > Hello Sharon,
> >
> > have you tried tissue from autopsies? Standard specimen taken from
> > organ are often quite alright. I have used them in seminars for
last year
> > students.
> >
> > Alexander Brands, M.D.
> > Institue of Toxicology
> > University of Tuebingen
> > Germany
> Hi  Alexander,
> Yes I have tried autopsy material several times. The result:TERRIBLE.
> I have aways found the preservation to be hopeless because of PM
> interval.
> Sharon Marshall
> Anatomy & Cell Biology
> ----------------------------------------------------------------------
> Date: 24 Feb 1999 02:56:53 -0600
> From: "Sarah A. Jones" <>
> Subject: "Culture Committee" revisited (summary)
> Since so many of you requested it, I am sending the suggestions:
> 1)This could be fun for all.
> I work for the Federal Government and we have to have some cultural
> for our EEO.  We have done a pot luck for Cinco de Mayo and other
> holidays.  Of course at our lab food is always an attractant.  There
> also videos about different people, events, etc from a variety of
> - - you can have popcorn and video one afternoon.
> I hope this helps.  Good luck and have fun with it - it is really
> interesting to learn about different cultures.
> 2) What you do will depend on the level of co-operation you can expect
> from your people. If it is a large group it is almost impossible to
> get more than 50% compliance in any event you may want to organise.
> But here are some thing that we have tried. I am a food/ outdoor
> person so dont be surprised if this is what I favour.
> Why not try a weekend away? We have had, for the last 2 years an
> informal pathology meeting where 2 sister departments from different
> cities meet at a halfway point. This way you can organise some
> academic meetings, games and/or other outdoor activities. It is
> an ideal way to combine relaxation with getting to know one
> another.
> Another outdoor option is the so called "team building"
> exercise- where you all go out and do something adventurous such as
> whitewater rafting. This requires at least someone with some
> expertise in the activity, but there are companies that specialise in
> organising such events.
> More simplistically, "dinner or tea"  with a regional or ethnic theme
> if you are a cosmopolitan group, will go a long way
> to get people to socialise and commit themeselves to an activity
> 3) Try taking "recess" together and go outside for a walk or hike up
> stairs a
> few times a week. A fun way to encourage certain behaviors is to
hand out
> candy bars or fast food gift certificates if people meet a goal (no
> that week, work out by a certain time all week,etc) What really can
pull a
> team together is to adopt a charity - maybe a family or a particular
> activity like Humane Society - something that everyone can work on
just a
> little but the results can be important. We recycle all our pop cans
and use
> the money actually for lab treats but we've talked about giving a
> amount to a charity - can't agree on which one - so if you're going
to do a
> charity of any kind it's got to be one everyone voted on and agrees
> 4) I am a supervisor of 10 histotechs and 2 clerks.  We try to have
> potlucks with a theme.  This year we had a "blue moon" brunch and a
> Valentine's Day party.  At the end of the year, we have a guess the
> number of blocks contest.  We also had a contest to name our new
> processor.  When someone has done an exceptionally fine job, I will
> e-mail the rest of the group that a certain tech has been named
"tech of
> the week". We decorate for all the holidays.  Sometimes I will furnish
> food for our monthly meetings.
> I know it is difficult to keep people upbeat and it is especially
> difficult if you have any "whiners".  A little break from the normal
> routine does seem to help.
> 5) One thing that we've done here in my department, is we celebrate
> ones
> birthday.  We do this by having a cake every month.  Of course,
> and acknowledging ones birthday is personal, so it is by volunteer
> that they give the okay for administration to publish and print (on
> cake) whose birthday it is that month.  We take pictures, do the happy
> birthday song and all.  The pictures are put in an album and left in
> staff lounge for all to share.
> To help with the cost,  all the lab section supervisors contribute
for the
> cakes.
> So far we have had a good response and no one is left out (unless by
> choice).
> 6) These are activities we have done during Lab Week celebrations.
> Last year we had a chili cookoff and the winner got a $100 gift
> to a very nice restaurant. A vendor donated the prize and there were
> entries to feed the lab for lunch. I don't know if this would work
for you
> because when I hear "regional", I am wondering if everyone is in the
> place.
> A very popular one, as you remember, was matching baby pictures with
> employee. We also did this with parents and this year we are doing
it with
> feet. I will be taking a picture of one foot from all who wish to
> participate. Many people think this will be fun but said they will
get a
> pedicure first! The picture goes on a board and each entrant tries
to match
> the foot to the right person.
> Another popular one: We bought about 80 Lab Week refrigerator
magnets and
> hid them in all areas of the lab. You can imagine all the places we
came up
> to hide them with the Haller furniture being metal! The magnets were
> in for gifts, with some magnets having a special mark which meant that
> person got a special gift.
> This year we are giving everyone a gift - a pack of post-it notes
that say
> on the bottom....from a valued member of the EMC Laboratory Services
> 7) One of the contests you might consider is to place seveal
> different sized tissue sections on a water bath. and set point
values on
> each section the person that can put the most sections neatly on a
> wins. I have found there are not to many people that have the hand eye
> dextarity to perform this simple task unless you work with it evey
day. The
> tiny 7mm sections should have the highest value. I think this is the
size of
> our small cassettes. Make sure the water bath is filled to the max
> sectons leaving very little room to manuver. It may not be a great
idea for
> a contest but it is cheap.
> 8) When I was at another institution, I was on the Lab Activities
> anyway, they called me "cruise director" and we had potluck dinners
> times a year, sometimes with an ethnic theme (everyone brings some
dish that
> is from whatever country or part of the US they are from) sometimes
it was a
> "spring" theme (usually in the spring) with things you think of more
in warm
> weather....salads, cold dishes, etc., sometimes it was a chili
"cookoff" or
> whatever kind of competetion (judges have to be diplomats in that
case) and
> we usually took up money from the pathologists (who didn't have to
> unless they wanted to) and bought a cooked turkey or ham or
> one time I bought shrimp with the money and cooked scampi for
> food is always good!!  Seasonal door decorating contests are always
> organize an after work softball game... or team to play in a league
in your
> area..... those were always good... a Friday after work "Happy Hour"
with or
> without some local restaurant is good for those who are
> interested.... a t-shirt decorating contest limited to reagents and
> found in the department is good.... an ID the baby photo contest with
> inexpensive prizes is good...... work together to do a departmental
> cookbook..... if you're big enough, have it professionally printed,
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