Re: Daily Digest
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| From: | Steve & Dolores Townsend <townsend@erinet.com> (by way of histonet) |
| To: | histonet <histonet@magicnet.net> |
| Reply-To: | |
| Content-Type: | text/plain; charset="us-ascii" |
Please take me off your mailing list
tks
steve
----- Original Message -----
From: HistoNet Server <HistoNet@Pathology.swmed.edu>
To: HistoNet Server <HistoNet@Pathology.swmed.edu>
Sent: Friday, February 26, 1999 1:02 AM
Subject: Daily Digest
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 01:33:25 -0600
>From: "A G DU TOIT" <AGDT@GERGA.SUN.AC.ZA>
>Subject: Re: SHANDON LINISTAIN GLX
>
>Dear Kimberley,
>We have been using two of the Linistain stainers for the last almost
>ten years without any problems. On one of the machines we had to
>replace a gearbox while still under guarantee. They are working very
>nicely and I must say they are in daily use. So far we never had any
>problems with tissue coming lose from the slide. The temp is set at
>65 deg C. The time it takes for a slide to move from one reagent to
>the next is about 20 seconds.
>Hope this help a bit.
>Regards.
>Andre du Toit.
>Department of Anatomical Pathology
>Tygerberg Hospital.
>Cape Town
>South Africa
>
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 03:15:20 -0600
>From: "Sue.Hacker" <Sue.Hacker@bbsrc.ac.uk>
>Subject: Shandon Linistain
>
>Dear Kimberley,
>We used to use a Linistainer in my previous lab, we didn't have problems
>with sections coming off but then we did always dry the slides in a 60oC
>oven for about 15 mins prior to putting them on the stainer.
>Personally I loved the Linistainer much preferred it to the Varistain
>which is what we have now.
>By the way , on the subject of duplicate messages, I haven't had ANY, I
>am obviously one of the lucky ones ! .
>Sue Hacker,
>IAH.Compton.
>Berks.UK.
>
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 03:30:47 -0600
>From: "Marshall, Sharon, Mrs" <marshall@anat.uct.ac.za>
>Subject: Re: Messages duplicated
>
>> Date: Wed, 24 Feb 1999 01:39:16 -0800
>> From: "Sarah A. Jones" <hawkmoon15@earthlink.net>
>> Subject: Messages duplicated
>
>> Does anyone have any idea why messages sent to the Histonet are being
sent
>> in duplicate? I only sent mine once, and it has appeared again, 23
minutes
>> after it appeared the first time.
>>
>> Sarah A. Jones
>> <hawkmoon15@earthlink.net>
>>
>Hi Sarah,
>I have experienced the same problem. My messages as well as the ones
>I have been receiving are being duplicated quite a few times. I am
>also not sure why.
>Sharon Marshall
>Anatomy & Cell Biology
>UCT
>South Africa
>e-mail: marshall@anat.uct.ac.za
>>
>>
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 04:00:42 -0600
>From: Christof Henne <Christof.Henne@dakogmbh.de>
>Subject: Antibodies for paraffin
>
>Dear Mary,
>
>John Bliss already mentioned the CD56 from Zymed.
>Here are suggestions for CD4 and CD8:
>Novocastra/Vector offers a CD4 for Paraffin (Clone 1F6, # NCL-CD4-1F6)
>DAKO Corp. offers a CD8 for Paraffin (Clone C8/144B, # M 7103)
>
>Best regards
>Christof Henne
>DAKO Germany, Hamburg
>
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 04:30:58 -0600
>From: "Ms Louise Taylor" <179LOU@chiron.wits.ac.za>
>Subject: Neuraminidase-PTHLP
>
>Hi Histonetters
>
>Firstly I would like to thank everyone who replied to my querie
>concerning the IHC on mouse/rat tissue. It has helped.
>My other querie is on neuraminidase, the spec on the PTHPL antibody
>from Oncogene science Cat GF08. Is anyone using this antibody ?
>I would like to know what dilution to use the neuraminidase ( 1U =
>100ul) from Boehringer Mannheim Cat 269 611 ; as it is not suggested
>in the spec, as usual!!!! Any suggestions will be welcome.
>
>Thanks
>Zenobia Haffajee
>SAIMR
>Anatomical Pathology
>South Africa
>
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 05:00:33 -0600
>From: grazyna.wieczorek@pharma.Novartis.com
>Subject: Re: CD4, CD8, CD56 - human paraffin
>
>Dear Mary,
>
>I tried (successfully) anti-CD56 (NCAM) on human tissue (paraffin sections)
>from Novocastra (NCL-CD56-1B6).
>Pretreatment: microwave cooking (95 degr. C 10 min) in citrate buffer pH
>6.0
>Antibody dil.: 1:50.
>
>Grazyna
>
>
>
>
>
>
>Mary Vaughan <mvaughan@sc3102.med.buffalo.edu> on 24.02.99 21:27:51
>
>To: HistoNet@Pathology.swmed.edu
>cc: (bcc: Grazyna Wieczorek/PH/Novartis)
>Subject: CD4, CD8, CD56 - human paraffin
>
>
>
>
>Good Afternoon All,
> Does anyone have CD4, CD8, CD56 [vs. human] working in paraffin
>sections?
> Best Regards,
> Mary Vaughan HT(ASCP)
> Roswell Park Cancer Institute
> Pharmacology + Therapeutics
> Elm + Carlton Sts. CDC-121
> Buffalo, NY 14263
>
>
>
>
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 07:00:29 -0600
>From: vandeplas@aurion.nl (Peter van de Plas)
>Subject: Re: antigen retrival
>
>>Cindy wrote:
>> I am going to be using microwave antigen retrival for a couple of
>>antibodies that I have not previously stained with. I was wondering
>>if anyone had any recipes for Citrate Buffers or any other
>>buffer solutions used for this purpose?
>>
>- -----------------------------------
>Dear Cindy,
>The optimal AR protocol differs from primary to primary.
>The review article by Shan-Rong Shi might be of help.
>Shi et al (1997) J. Histochem. Cytochem. 45, 327-343
>Peter
>
>========================================
>Peter van de Plas
>AURION
>Costerweg 5
>6702 AA Wageningen
>The Netherlands
>phone: (31)-317-497676
>fax: (31)-317-415955
>http://www.aurion.nl
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 07:00:58 -0600
>From: vandeplas@aurion.nl (Peter van de Plas)
>Subject: Re: IHC titer
>
>Verrucous wrote:
>> I would like some help with titering my immunohistochemical primary
>>antibodies, please.
>> How many dilutions and in what ranges. What does an economical pipette
for
>>this procedure cost? Do you use titer records & retain titer slides? Do
you
>>adjust antibodies on special request from the Pathologist to demonstrate
>>specifically ihoti markers?
>> Iive told my Pathologists that we need to invest in the pipette for
proper
>>titering procedure. I keep records of the dilutions we arrive at and make
the
>>Pathologist sign off on each lot of primary antibody.
>- ---------------------------------
>The dilution of a primary antibody that will give you the optimal signal to
>noise ratio depends on a lot of factors, e.g. concentration of your stock,
>binding affinity of the primary antibody and the detection system you are
>using. The following (my approach) might be of help:
>
>In general you incubate with a primary antibody concentration between 10
>and 1 ug/ml. When you have a high affinity antibody the concentration will
>be somewherebetween 1 and 0.1 ug/ml. When I do not have info on the
>concentration of the antibody I always start with a 10-fold dilution series
>and I test 4 different dilutions. With supernatants I use the dilution
>series 1/1-1/10-1/100-1/1000. For a serum I use 1/10-1/100-1/1000-1/10000.
>A negative control is always included. These first results will already
>give you an indication in what region the optimal dilution will be. For
>example 1/10 stains the whole section, 1/100 strong signal but still a lot
>of background, 1/1000 signal and no background, 1/10000 hardly any signal
>and no background. From this I conclude that the optimal dilution will be
>between 1/100 and 1/1000. In the second experiment I test a 2-fold dilution
>series with again 4 different dilutions: 1/200-1/400-1/800-1/1600.
>
>When an indication about the dilution is given (e.g. by the manufacturer) I
>start with the 2-fold dilution series as already described. E.g. when1/100
>in immunofluorescence is mentioned in the package insert I test
>1/50-1/100-1/200-1/400.
>
>Hope this is of help.
>Peter
>
>========================================
>Peter van de Plas
>AURION
>Costerweg 5
>6702 AA Wageningen
>The Netherlands
>phone: (31)-317-497676
>fax: (31)-317-415955
>http://www.aurion.nl
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 08:15:41 -0600
>From: "Tim Morken" <timcdc@hotmail.com>
>Subject: Re: IHC titer
>
>The best place to start is with any product info you get with the
>anitbody. Use the recommended dilution as a starting point and adjust it
>from there.
>
>If you don't have any info (as it usually is in our case) we do titers
>over a broad range to start with and then narrow the range as we gain
>information about the antibody.
>
>For instance, we might do 1:50, 1:500 and 1:1000 to start. Depending on
>the results, we would do the next titer series near one of those
>dilutions. For instance, if it works better at 1:500, our next series
>might be 1:300, 1:500, 1:700. From those results we sould narrow it
>further.
>
>Alternativly, you could do a doubling series in which you start at 1:20
>and then double the dilution up to 1:1000 or more all in one run.
>
>We generally start with digestion with Protienase K and only try antigen
>retrieval if no positive results are obtained.
>
>We keep records of the trials and keep the experiment that shows the
>best dilution as a reference. That experiment is noted in the antibody
>log.
>
>For hardware it would be best to have several pipets. They will cost you
>in the neighborhood of USD 150 to 250 each but they are well worth the
>cost since they allow you to make very small volumnes of expensive
>antibodies.
>
>We have several in the following ranges:
>0.5 to 10 ul
>10 to 100 ul
>100 to 1000 ul
>200 to 100 ul
>50 to 200 ul
>10 to 50 ul
>
>These are checked for calibration annually.
>
>Tim Morken, B.A., EMT(MSA), HTL(ASCP)
>Infectious Disease Pathology
>Centers for Disease Control
>MS-G32
>1600 Clifton Rd.
>Atlanta, GA 30333
>USA
>
>email: tim9@cdc.gov
> timcdc@hotmail.com
>
>FAX: (404)639-3043
>
>
>
>
>
>- ----Original Message Follows----
>Date: Wed, 24 Feb 1999 21:51:54 -0500 (EST)
>From: Verrucous@aol.com
>Subject: IHC titer
>To: HistoNet@Pathology.swmed.edu
>
>Hi Histoinans !
> I would like some help with titering my immunohistochemical primary
>antibodies, please.
> How many dilutions and in what ranges. What does an economical
>pipette =
>for
>this procedure cost? Do you use titer records & retain titer slides? Do
>yo=
>u
>adjust antibodies on special request from the Pathologist to demonstrate
>specifically =93hot=94 markers?
> I=92ve told my Pathologists that we need to invest in the pipette for
>p=
>roper
>titering procedure. I keep records of the dilutions we arrive at and
>make =
>the
>Pathologist sign off on each lot of primary antibody.
> Thank You for any help
> Respectfully
> Verrucous
>
>
>
>
>
>
>
>
>
>______________________________________________________
>Get Your Private, Free Email at http://www.hotmail.com
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 08:26:33 -0600
>From: "Tom & Beth Galati" <tgalati@shentel.net>
>Subject: Stop mailing these messages!
>
>If everyone would stop mailing the server saying we all received duplicate
>messages, we would not have to read 150 messages a day!
>
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 09:00:29 -0600
>From: mark.lewis@shandon.com
>Subject: RE: Shandon Correction
>
>CORRECTION !!!!!
>I apologize for a slip of the finger... the toll free number for both Mark
and
>Amy is......
>1-800-245-6212 exts. 4013 and 4026 respectively..
>
> And I'm still not used to the fact that Amy Lamp is now Amy Hindes....My
>apologies....
>
>:>)
>
>Mark Lewis
>
> ----------
>From: mark.lewis@shandon.com [SMTP:MIME :mark.lewis@shandon.com]
>Sent: Wednesday, February 24, 1999 6:38 PM
>To: kimberly_l.parker@winn.chcs.am; histonet@pathology.swmed.edu
>Subject: RE: SHANDON LINISTAIN GLX
>
><<File: ENVELOPE.TXT>>
> --------------------------------------------------------------------------
>--
>Kim,
>
>
>Have you called Shandon's Product Support line for Assistance? Please
>feel free to give us a call any time and we will work with you on this.
>There are two Technical Specialists that are available to help with
>product application problems; myself ( Mark Lewis ) and Amy Lamp.
>
>
>Our toll free number is:
>1-800-245-6212 ext. 4013 - Mark Lewis
>1-800-245-6213 ext. 4026 - Amy Lamp
>
>
>
>
>Thanks,
>
>
>Mark Lewis
>Technical Specialist
>Shandon
>1-800-245-6212 ext. 4013
> ----------
>From: kimberly_l.parker@winn.chcs.am [SMTP:PC
>:kimberly_l.parker@winn.chcs.amedd.army.mil]
>Sent: Wednesday, February 24, 1999 1:02 PM
>To: kimberly_l.parker@winn.chcs.am; histonet@pathology.swmed.edu
>Subject: SHANDON LINISTAIN GLX
>
>
><<File: ENVELOPE.TXT>>
>
>
> --------------------------------------------------------------------------
>
> --
>DOES ANYONE USE THIS STAINER? I JUST INSTALLED ONE HERE AND I AM HAVING
>TROUBLE WITH KEEPING TISSUE ON THE SLIDE AND SETTING UP THE STAIN. THE
>HEATING MECHANISM IS AT 70 AND I DON'T THINK IT GETS ANY HOTTER, AND ITS
>NOT
>PROGRAMMABLE AS FAR AS STAINING TIMES OR HEATING TIMES.
> KIM PARKER
>WINN ARMY HOSPITAL
>FT.STEWART GA 31314
>(912)370-6083
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 09:30:17 -0600
>From: alexis.a.schulman.1@nd.edu
>Subject: indirect immunofluorescence protocol
>
>Hi! I am collecting protocols to try out indirect IHC using goat-anti-mouse
>fibrinogen as a primary ab and FITC labelled rabbit-anti-goat as my
>secondary in frozen sections. Any suggestions?
>Thanks in advance,
>
>Alexis
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 09:31:04 -0600
>From: "Nocito, Joseph" <joseph_nocito@srhc.iwhs.org>
>Subject: RE: antigen retrival
>
>Cindy,
>I have a receipe that I learned about at the NSH meeting in Salt Lake city
>last year.
> Citrate buffer pH 6.0
>Citric acid 0.1M
> citric acid monohydrate.............. 21.01 gm
> distilled water........................ 1000 mL
>
>Sodium Citrate 0.1M
> sodium citrate dihydrate............. 29.41 gm
> distilled water........................ 1000 mL
>
>Working Solution
> 0.1M citric acid.................. 9.0 mL
> 0.1M sodium citrate.......... 41.0 mL
> distilled water.................. 450.0 mL
>
>adjust the pH with either 1N sodium hydroxide or 1N hydrochloric acid.
>
>This is good for general use. However, some antibodies require a higher pH
>buffer. It's trial and error Hope this helps.
>
>Joe Nocito, B.S., HT(ASCP)QIHC
>Histology Supervisor
>Christus Santa Rosa Hospitals
>San Antonio, Texas
>
>> -----Original Message-----
>> From: vandeplas@aurion.nl [SMTP:vandeplas@aurion.nl]
>> Sent: Wednesday, February 24, 1999 6:59 PM
>> To: Histonet@Pathology.swmed.edu
>> Subject: Re: antigen retrival
>>
>> >Cindy wrote:
>> > I am going to be using microwave antigen retrival for a couple of
>> >antibodies that I have not previously stained with. I was wondering
>> >if anyone had any recipes for Citrate Buffers or any other
>> >buffer solutions used for this purpose?
>> >
>> -----------------------------------
>> Dear Cindy,
>> The optimal AR protocol differs from primary to primary.
>> The review article by Shan-Rong Shi might be of help.
>> Shi et al (1997) J. Histochem. Cytochem. 45, 327-343
>> Peter
>>
>> ========================================
>> Peter van de Plas
>> AURION
>> Costerweg 5
>> 6702 AA Wageningen
>> The Netherlands
>> phone: (31)-317-497676
>> fax: (31)-317-415955
>> http://www.aurion.nl
>>
>>
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 09:31:34 -0600
>From: "Mark Elliott" <MElliott@prl.pulmonary.ubc.ca>
>Subject: CD4 and CD8
>
>Mary Vaughn was asking about CD4 and CD8 working on paraffin-embedded human
>tissue. I am working with human lung samples which are formalin-fixed and
>paraffin
>embedded, using the NovaCastra antibodies (NCL-CD4-1F6 and NCL-CD8-4b11)
with
>not much problem. I autoclave the sections for 7 minutes in 1mM EDTA ph8.0
>and then
>do an APAAP method using the primaries at 1/50 dilution of each. I use a
>AS-BI
>phosphate/New Fuchsin substrate so I can clear in xylne/Hemo-De and mount
with
>
>Entellan. The only problem I have been having is some of the tissue (from
>another
>institution) keeps falling off in the autoclave, but because of suggestions
>made earlier
>on the Histonet I am trying to coat the slides with Parlodion/Celloidin to
>keep them on
>and it seems to be working, just needs a little more tweaking. If anyone
>wants furhter
>info please contact me.
>
>
>Mark Elliott, PhD
>Research Associate
>UBC-Pulmonary Research Lab
>St. Paul's Hospital
>Vancouver, BC Canada V6Z 1Y6
>604-631-5351 (FAX)
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 10:06:23 -0600
>From: "Mary L. Verlinde" <verlinde@ahdlms.cvm.msu.edu>
>Subject: Peel-away
>
>
>I am looking for the catalogue number for peel-away made by Sakura. Thanks
>in advance.
>
>
>
>Mary Verlinde
>AHDL
>Mich. State Univ.
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 10:07:13 -0600
>From: "Goodwin, Diana" <DGoodwin@CHSNJ.org>
>Subject: Commercial controls for ER/PR
>
>Hello, Histoland.
>
>I am working with another tech who does quantitations on ER/PR sections
>using image analysis. Does anyone know of a source for controls that
>would demonstrate low-end, midrange and high-end positivity? It would
>be great if they were all on one slide, but any info will be greatly
>appreciated.
>
>Thanks!
>
>Diana Goodwin, HT
>Trenton, NJ
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 10:33:46 -0600
>From: Katie B <bresee98@yahoo.com>
>Subject: JB4 Microtome
>
>Forgive if this is a duplicate, but I don't think my first one went
>through:
>
>Was wondering if anyone out there has a used Sorval JB4 Microtome that
>they are interested in selling. Or, does anyone know where I may
>inquire to buy one?
>
>==
>Catherine "Katie" Bresee Bennett
>Laboratory for Experimental Pathology
>Department of Veterinary Pathology
>Michigan State University
>
>*new* e-mail: bresee98@yahoo.com
>
>_________________________________________________________
>DO YOU YAHOO!?
>Get your free @yahoo.com address at http://mail.yahoo.com
>
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 10:49:23 -0600
>From: Sharyn S Caplan <phinny1@juno.com>
>Subject: Storage of control blocks and slides
>
>
>Hi Fellow Histonetters
>
>I am presently preparing for a CAP inspection, and need some input.
>At my last position I stored some of my
>immuno control slides in the refrigerator and/or freezer to help with
>viability. I had been given this advise from several techs that had
>been doing immunos even longer than myself. Examples of these were ER,
>PR, Her 2, Hepatitis,p53.
>When I suggested this practice to be included in our protocols, my new
>pathologist said he never heard of this practice, thought it was not
>necessary and asked me for data supporting it. Since I am in Florida
>now, where heat and humidity can be a problem, I am concerned. If
>anybody knows of any articles that either support or negate this idea,
>please give me a heads-up. I would be ever so greatful.
>
>Thanks
>Sharyn Caplan
>
>Sharyn1678@yahoo.com
>___________________________________________________________________
>You don't need to buy Internet access to use free Internet e-mail.
>Get completely free e-mail from Juno at http://www.juno.com/getjuno.html
>or call Juno at (800) 654-JUNO [654-5866]
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 11:05:36 -0600
>From: "Anna Magallanez" <MGLLNA@powell.fabrik.com>
>Subject: Fwd:subscribe digest
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 11:06:05 -0600
>From: vandeplas@aurion.nl (Peter van de Plas)
>Subject: Re: Peel-away
>
>I can only give you the catno. in the cataloque of Electron Microscopy
>Sciences:
>19300 Peel-away paraffin 53-55 oC
>19302 Peel-away paraffin 56-58 oC
>19304 Peel-away paraffin 60-62 oC
>70180 embedding mold truncated 8x8mm
>70181 embedding mold truncated 12x12mm
>70182 square mold 22x22mm
>70183 rectangular 22x30mm
>70184 rectangular mold 22x40mm
>Might be of help.
>Peter
>
>========================================
>Peter van de Plas
>AURION
>Costerweg 5
>6702 AA Wageningen
>The Netherlands
>phone: (31)-317-497676
>fax: (31)-317-415955
>http://www.aurion.nl
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 12:15:23 -0600
>From: "John Spair" <jspair@multicare.com>
>Subject: Fw: Formalin/Alcohol Question
>
>
>Histonetters:
>
>Some people in pathology say they have experienced a loss of
>immunoreactivity when tissues are fixed in formol-alcohol. One lab for
>instance that we have referred out ICC, won't do ICC on any tissue fixed in
>formol-alcohol. Another lab says they have no trouble whatsover. Recently
>our pathologists started fixing our breast and colon cases in
formol-alcohol
>and since most of these breast cases get a "breast battery" panel done on
>them, such as ER/PR, etc. - a concern has arisen between our pathology
group
>about the use of formol-alcohol.
>
>I'm curious to find out other histonetters opinions, or experiences before
>making a final decision and telling our pathology group that we cannot use
>it any longer. Thank you.
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 13:01:17 -0600
>From: "Nocito, Joseph" <joseph_nocito@srhc.iwhs.org>
>Subject: RE: Formalin/Alcohol Question
>
>John,
>our experience with formalin substitutes such as alcoholic formalin and
zenk
>formalin have not been good. We have a tendency to get increased
>non-specific background staining.
>
>> -----Original Message-----
>> From: John Spair [SMTP:jspair@multicare.com]
>> Sent: Thursday, February 25, 1999 12:10 PM
>> To: histonet@pathology.swmed.edu
>> Subject: Fw: Formalin/Alcohol Question
>>
>>
>> Histonetters:
>>
>> Some people in pathology say they have experienced a loss of
>> immunoreactivity when tissues are fixed in formol-alcohol. One lab for
>> instance that we have referred out ICC, won't do ICC on any tissue fixed
>> in
>> formol-alcohol. Another lab says they have no trouble whatsover.
>> Recently
>> our pathologists started fixing our breast and colon cases in
>> formol-alcohol
>> and since most of these breast cases get a "breast battery" panel done on
>> them, such as ER/PR, etc. - a concern has arisen between our pathology
>> group
>> about the use of formol-alcohol.
>>
>> I'm curious to find out other histonetters opinions, or experiences
before
>> making a final decision and telling our pathology group that we cannot
use
>> it any longer. Thank you.
>>
>>
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 13:12:41 -0600
>From: "John Spair" <jspair@multicare.com>
>Subject: Fw: Formalin/Alcohol Question
>
>
>- -----Original Message-----
>From: John Spair <jspair@multicare.com>
>To: histonet@pathology.swmed.edu <histonet@pathology.swmed.edu>
>Date: Thursday, February 25, 1999 10:09 AM
>Subject: Fw: Formalin/Alcohol Question
>
>
>>
>>Histonetters:
>>
>>Some people in pathology say they have experienced a loss of
>>immunoreactivity when tissues are fixed in formol-alcohol. One lab for
>>instance that we have referred out ICC, won't do ICC on any tissue fixed
in
>>formol-alcohol. Another lab says they have no trouble whatsover.
Recently
>>our pathologists started fixing our breast and colon cases in
>formol-alcohol
>>and since most of these breast cases get a "breast battery" panel done on
>>them, such as ER/PR, etc. - a concern has arisen between our pathology
>group
>>about the use of formol-alcohol.
>>
>>I'm curious to find out other histonetters opinions, or experiences before
>>making a final decision and telling our pathology group that we cannot use
>>it any longer. Thank you.
>>
>>
>
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 13:45:33 -0600
>From: Robert.Lott@bhsala.com
>Subject: SHANDON LINISTAIN GLX
>
>
>Kim,
>If you would like to talk with me directly, I think I can help...
>We have two of GLX's, and I believe they are optimized for producing ideal
>H&Es.
>
>Robert Lott, HTL(ASCP)
>Baptist Health System
>Birmingham, AL
>205-592-5388
>Robert.Lott@bhsala.com
>- ------------------( Forwarded letter 1 follows )--------------------
>Date: Wed Feb 24 11:52:37 1999
>To: kimberly_l.parker@winn.chcs.amedd.army.mil
>Sender: HistoNet@Pathology.swmed.edu
>Subject: SHANDON LINISTAIN GLX
>
>DOES ANYONE USE THIS STAINER? I JUST INSTALLED ONE HERE AND I AM HAVING
>TROUBLE WITH KEEPING TISSUE ON THE SLIDE AND SETTING UP THE STAIN. THE
>HEATING MECHANISM IS AT 70 AND I DON'T THINK IT GETS ANY HOTTER, AND ITS
NOT
>PROGRAMMABLE AS FAR AS STAINING TIMES OR HEATING TIMES.
>
>KIM PARKER
>WINN ARMY HOSPITAL
>FT.STEWART GA 31314
>(912)370-6083
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 13:59:15 -0600
>From: Robert.Lott@bhsala.com
>Subject: Retic stain(s)
>
>
>Dear M.
>Retic.stains are not difficult and the Gomori method is a great
technique...
>but you "MUST" remember one important factor. Inconsistent results are
>caused by the way the "ammoniacal silver solution" is made. The
sensitivity
>of staining is controlled by the balance between ammonia and silver nitrate
in
>this solution and you "MUST" be careful not to add too much ammonia when
>clearing the solution. Stop short of a clear solution and err on the side
of
>"dirty" if you know what I mean. This is the biggest mistake histotechs
make
>when making ammoniacal silver. Filter the "dirty" solution and proceed.
>Livermakes the best control for retic stains.
>
>I have assumed that your oxidizer, sensitizer and reducing solution are all
>fresh!
>
>Robert Lott, HTL(ASCP)
>Baptist Health System
>Birmingham, AL
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 14:31:58 -0600
>From: FreidaC@aol.com
>Subject: Re: Retic stain(s)
>
>Old ammonia can also give you problems with the reticulum stain. Be sure
you
>are using a reasonably fresh solution. If the bottle has a lot of head
space,
>or the solution level is down very far, it probably has lost strength. You
>should go to a fresh bottle. Buy the smallest bottles possible.
>
>Personally, I like the Gordon and Sweets technique best.
>
>Freida Carson
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 14:42:13 -0600
>From: "O'Neil, David B." <David.O'Neil@nrc.ca>
>Subject: microtome parts, etc.
>
>I am looking for some parts for our histology setup. I have quotes from
the
>manufacturers, but not being familiar with what the marketplace has to
>offer, I thought this forum might produce 3rd party or alternative
suppliers
>for these pieces.
>
>1. Quick release jaw clamp for Tissue Tek cassettes for Zeiss HM355
>Microtome (or one that could be adapted)
>2. Disposable Knife holder for above
>3. Plate for Shandon Autosharp knife sharpener (reconditioned)
>
>Suppliers feel free to contact me directly. Thanks.
>
>David O'Neil tel: (902) 426-8258
>National Research Council of Canada fax: (902) 426-9413
>Institute for Marine Biosciences
>1411 Oxford St.
>Halifax, Nova Scotia B3H 3Z1
>Canada
>email: david.o'neil@nrc.ca
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 15:00:59 -0600
>From: "Klemme, Nancy" <nancy.klemme@sakuraus.com>
>Subject: Re:Peel-away
>
>I do not know who the manufacturer of "Peel-A-Way" paraffin is. I have,
>however, seen it available in the Shandon Catalog. The toll-free number
for
>Shandon is 800-547-7429.
>
>Nancy Klemme, HT(ASCP)
>Customer/Product Support Mgr.
>Sakura Finetek USA, Inc.
>1750 West 214th Street
>Torrance, CA 90501
>
>Web Page = www.sakuraus.com
>e-mail = nancy.klemme@sakuraus.com
>Phone = 800/725-8723
>
>____________________Reply Separator____________________
>Subject: Peel-away
>Author: "Mary L. Verlinde" <verlinde@ahdlms.cvm.msu.edu>
>Date: 2/25/99 10:46 AM
>
>From: Mary L. Verlinde
>Date: Thu, Feb 25, 1999 10:46 AM
>Subject: Peel-away
>To: 'HISTONET@PATHOLOGY.SWMED.EDU'
>
>I am looking for the catalogue number for peel-away made by Sakura. Thanks
>in advance.
>
>
>
>Mary Verlinde
>AHDL
>Mich. State Univ.
>
>
>- ----------
>Received: from email.swmed.edu by powell.fabrik.com
> with SMTP (Fabrik F07.3-000)
> id SINN.12427763@powell.fabrik.com ; Thu, 25 Feb 1999
08:46:34 -0800
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> nancy.klemme@sakuraus.com; Thu, 25 Feb 1999 10:45:38 CST
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> (Eudora Internet Mail Server 1.3.1); Thu, 25 Feb 1999 10:11:56 -0600
>Date: Thu, 25 Feb 1999 10:46:00 -0800 (PST)
>From: "Mary L. Verlinde" <verlinde@ahdlms.cvm.msu.edu>
>Subject: Peel-away
>To: "'HISTONET@PATHOLOGY.SWMED.EDU'" <HISTONET@PATHOLOGY.SWMED.EDU>
>Message-id: <36D59A77@ahdlms.cvm.msu.edu>
>
>- ----------
>
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 15:14:05 -0600
>From: "Bill Sinai (Anatomical Pathology)" <Bills@icpmr.wsahs.nsw.gov.au>
>Subject: Re: Retic stain(s)
>
>Date: Thu, 25 Feb 1999 14:05:01 -0600
>From: Robert.Lott@bhsala.com
>Subject: Retic stain(s)
>To: histonet@pathology.swmed.edu
>
>
>Dear M.
>Retic.stains are not difficult and the Gomori method is a great
technique...
>but you "MUST" remember one important factor. Inconsistent results are
>caused by the way the "ammoniacal silver solution" is made. The
sensitivity
>of staining is controlled by the balance between ammonia and silver nitrate
in
>this solution and you "MUST" be careful not to add too much ammonia when
>clearing the solution. Stop short of a clear solution and err on the side
of
>"dirty" if you know what I mean. This is the biggest mistake histotechs
make
>when making ammoniacal silver. Filter the "dirty" solution and proceed.
>Livermakes the best control for retic stains.
>
>I have assumed that your oxidizer, sensitizer and reducing solution are all
>fresh!
>
>Robert Lott, HTL(ASCP)
>Baptist Health System
>Birmingham, AL
>
>Dear all,
>I agree whole heartedly with Robert that Gomori Reticulin technique
>is great, but the ammonia:silver ratio is critical and the staining
>results previously described are definitely due to the "quality" of
>the ammoniacal silver solution. If you have someone in your lab who
>consistently makes it correctly NEVER let them go. My mentor a PhD
>in histochemistry always said the time to stop dissolving the
>precipitate was when there were approx. 20 grains left in the bottom.
>Then invert the stoppered measuring cylinder 20 times before adding
>any more ammonia (if required). There is always a little excess
>ammonia on the sides of the container and this most often completes
>the process.
>Too much ammonia can also be the cause of sections floating away
>during the final steps in the Gomori procedure.
>
>Good luck
>All the best in Histotechnology
>Bill Sinai
>Department Manager
>Tissue Pathology
>ICPMR Westmead Hospital
>WESTMEAD NSW AUSTRALIA
>Phone 61+2+9845 7774 Fax 61+2+9687 2330
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 16:31:09 -0600
>From: Louise Burrell <lburrell@pathbox.wustl.edu>
>Subject: Re: Daily Digest
>
>Dear everyone- I have been gone for a week so here goes.
>Gayle Callis is, of course, right on re: chrome alum gelatin subbed
>slides---alos if you get a background and you are doing AgNO3 staining-
>Silver-You can inse slide gently AND quickly in a dilute( 10 drops to 400
>mls H2O) solution on NH4OH --ammonia water----then wipe any excess around
>tissue section off with dilute solution prior to running down thru dist
>h2o; etohs and xylenes .
>Also, on the Gomori'S Retic--you musdt filter solution thru filter paper
>over slides slowly---if you lay them on a staining rack-horizontal- you
>can then rotate the rack gently forward and backwards----thjat always
>solved my problems----also-don't forget to refrigerate the agno3
>solutions.
>Double plus slides may be purchased from Fisher.
>The large slides are best and most easily purchased thru Brain Research
>lLabs--their # has been shoen here often--ask for Nora.They really help
>with any situation.
>To the gal who needs fresh-formalin grossly cut tissue---to trim etc. BY
>HERSELF---I have skin and am awaiting the rest for you.
>To anyone who needs a great Gallyas stain call 314 362 7421 and ask for
>Deborah---she has a modification to the Braak &raak method.
>To anyone who needs a great neurofibrillary/senile plaque stain- I gave
>the reference two weeks ago--will again if need be----one id for tangles
>and one is better for plaques---They are my modifications of the tried and
>true UCLA Modification of the Bielschowsky stain.
>
>
>
> With warmest regards,
> Louise "Beezie" Burrell
> Chief Technologist-Cancer Center
> Tissue Procurement Core Facility
> Campus Box #8056
> Washington University School of Medicine
> St. Louis, MO. 63110
> Ph: 314-454-7615
> Fax: 314-454-5525
>
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 16:31:48 -0600
>From: Phyllis Davie <pdavie@phenopath.com>
>Subject: Re: Fw: Formalin/Alcohol Question
>
>John,
> We have done many, many cases fixed in alcoholic formalin with our
>"breast battery", and we have no more problems with them than is typical.
> You can never guarantee that you will not get sporadic cases that have
>been over- or under-fixed, or that you will never have processing
>problems. But in general, the antibodies we use for breast cases (ER,
>PR, p53, Ki-67, and her-2-neu) all work well in that fixative, as long as
>the tissue is well-fixed and processed. If you have any specific
>questions, I'd be happy to discuss them with you, either on or off-line.
>
>
>>
>>Histonetters:
>>
>>Some people in pathology say they have experienced a loss of
>>immunoreactivity when tissues are fixed in formol-alcohol. One lab for
>>instance that we have referred out ICC, won't do ICC on any tissue fixed
in
>>formol-alcohol. Another lab says they have no trouble whatsover.
Recently
>>our pathologists started fixing our breast and colon cases in
formol-alcohol
>>and since most of these breast cases get a "breast battery" panel done on
>>them, such as ER/PR, etc. - a concern has arisen between our pathology
group
>>about the use of formol-alcohol.
>>
>>I'm curious to find out other histonetters opinions, or experiences before
>>making a final decision and telling our pathology group that we cannot use
>>it any longer. Thank you.
>>
>>
>>
>
>
>Phyllis Davie
>Clinical Laboratory Supervisor
>PhenoPath Laboratories
>Seattle, WA
>pdavie@phenopath.com
>(206)374-9009 phone
>(206)374-9009 fax
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 16:53:53 -0600
>From: Phyllis Davie <pdavie@phenopath.com>
>Subject: Re: IHC titer
>
>I'll address the question that I haven't seen answered yet. I do keep
titer
>records, but I don't retain titer slides. (I'd be drowning in them by
now!)
>I do, however, keep 1 or 2 examples of "good" staining on each antibody. I
>have a separate alphabetical file for these, and I have found it to be
>invaluable for times when I feel that the antibody has "drifted", or for
>rarely used antibodies, or when I have visitors who want to see what a
>specific antibody should look like, or when my boss wants to take a picture
of
>"just one good example of ......(whatever).
>
>>Hi Histoinans !
>> I would like some help with titering my immunohistochemical primary
>>antibodies, please.
>> How many dilutions and in what ranges. What does an economical pipette
for
>>this procedure cost? Do you use titer records & retain titer slides? Do
you
>>adjust antibodies on special request from the Pathologist to demonstrate
>>specifically ihoti markers?
>> Iive told my Pathologists that we need to invest in the pipette for
proper
>>titering procedure. I keep records of the dilutions we arrive at and make
the
>>Pathologist sign off on each lot of primary antibody.
>> Thank You for any help
>> Respectfully
>> Verrucous
>>
>>
>>
>
>
>Phyllis Davie
>Clinical Laboratory Supervisor
>PhenoPath Laboratories
>Seattle, WA
>pdavie@phenopath.com
>(206)374-9009 phone
>(206)374-9009 fax
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 17:32:57 -0600
>From: RCHIOVETTI@aol.com
>Subject: Re: microtome parts, etc.
>
>In a message dated 99-02-25 16:08:31 EST, David.O'Neil@nrc.ca writes:
>
><< I am looking for some parts for our histology setup. I have quotes from
>the
> manufacturers, but not being familiar with what the marketplace has to
> offer, I thought this forum might produce 3rd party or alternative
suppliers
> for these pieces.
> >>
>
>David,
>
>I'm sure you will get some responses from this listserver. If not, there
is
>quite a lot of traffic for used and spare parts on a couple of the
internet's
>Usenet Newsgroups. Two of the best are:
>
>sci.med.pathology
>sci.techniques.microscopy
>
>You can browse and post via your Newsgroup reader, or via Deja News'
website
>(www.dejanews.com) or Liszt's website (www.liszt.com).
>
>Happy Hunting!
>
>Bob
>******************************
>Robert (Bob) Chiovetti, Ph.D.
>President
>Microimaging Technologies, Inc.
>Tucson, Arizona USA
>Tel./Fax (520) 546-4986
>rchiovetti@aol.com
>Manufacturers' Representatives
>Systems Integrators
>Analog & Digital Imaging Systems
>Clinical & Research Microscopy
>Cytology/Histology/Pathology/EM
>*******************************
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 18:30:35 -0600
>From: larisonk@UONEURO.uoregon.edu
>Subject: plastic embedding of charcoal
>
>Histonetters,
>
>Here's a question to ponder:
>
>A graduate student in the archeology department has a collection of charred
>wood samples that she has recovered from various sites on Easter Island.
She
>is attempting to embed them and section them in hopes of identifying the
wood.
>
>She is currently embedding these samples in Spurrs, but hasn't had very
much
>luck cutting them because of incomplete infiltration. I suspect that part
of
>her problem is she is trying to process these things by hand using a vacuum
>dessicator. But is Spurrs the best thing to embed in?
>
>Karen Larison -- University of Oregon
>
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 18:31:22 -0600
>From: TABrecken@aol.com
>Subject: (no subject)
>
>
>In a message dated 2/15/99 2:42:11 PM Central Standard Time, TABrecken
writes:
>
><< Subj: FSH State Meeting
> >>
>
>
>
>
>******************* NOTE *******************
>There may be important message content
>contained in the following MIME Information.
>********************************************
>
>
>- ------------------ MIME Information follows ------------------
>
>This is a multi-part message in MIME format.
>
>- --part0_919987591_boundary
>Content-ID: <0_919987591@inet_out.mail.aol.com.1>
>Content-type: text/plain; charset=US-ASCII
>
><<<<<< See above "Message Body" >>>>>>
>
>- --part0_919987591_boundary
>Content-ID: <0_919987591@inet_out.mail.aol.com.2>
>Content-type: message/rfc822
>Content-transfer-encoding: 7bit
>Content-disposition: inline
>
>From: TABrecken@aol.com
>Return-path: <TABrecken@aol.com>
>To: Histonet@pathology.swmed.edu
>Subject: FSH State Meeting
>Date: Mon, 15 Feb 1999 15:42:11 EST
>Mime-Version: 1.0
>Content-type: text/plain; charset=US-ASCII
>Content-transfer-encoding: 7bit
>
>Florida Society for Histotechnology State Meeting
>April 30-May 2, 1999
>Embassy Suites Deerfield Beach Resort, Deerfield Beach, Florida
>1-800-EMBASSY for hotel reservations. Group ID HIS
>Room Rates: Ocean View $130 / Sunset View: $110
>
>Program
>
>Friday, April 30, 1999
>
>AM
>
>1. Microtomy: Yesterday, Today, and Tomorrow
> Bonnie M. Cohen, B.S.,HT(ASCP)HTL, Jackson Memorial Hospital
>
>2. Introduction to Paleopathology
> Harvey Saskin, M.D., Univ. of Miami Dept. of Pathology
>
>PM
>
>3. Future of Microtomy
> Susan Vrabel, B.A.,HT(ASCP)QIHC, Leica Microsystems
>
>4. Forensic Pathology
> Mike Bell, M.D., Dade County Medical Examiner Office
>
>Saturday, May 1, 1999
>
>AM
>
>5. Chemical & Biological Hazards in the Histology Lab
> Michael Rice, CT,HT(ASCP), Jackson Memorial
>
>6. Benefits of Epitope Retrieval in Immunohistochemistry
> Nancy Shellhorn, B.A.,HT(ASCP), Zymed Laboratories
> Tonia Breckenridge, A.A.,HT(ASCP), Baptist Hospital
>
>7. Diagnostic Electron Microsopy
> Rebecca Garrison, B.S.,EMT(MSA), University Medical Center
>
>PM
>
>8. Distance Education Via Web Courses: Will It Work in Histology?
> Merry Carter, MEd, MT(ASCP)SC, Florida Community College
>
>9. FNA's, Microbiology, & Histology: How It All Fits Together & AIDS Update
> Joseph Chaio, M.D., University Medical Center
>
>10. Current Trends in Immunohistochemistry
> B.J. Kearns, Biogenix
>
>Sunday, May 2, 1999
>
>11. Unlock the Secrets of Frozen Sections!
> Mequita Praet, HT(ASCP)
>
>12. Management Through the Dynamics of Team Performance
> Nancy Shellhorn, B.A.,HT(ASCP), Zymed Laboratories
> Joni Huff, A.S.,HT(ASCP), University Medical Center
>
>13. AIDS Update '99 1 Hour Only
> Joseph Chiao, M.D., University Medical Center
>
>All classes except #13 are (3.0) CEU.
>
>Friday Evening, Biogenix & Ventana Medical Systems will be hosting
Hospitality
>Suites. Room numbers will be posted at the Registration Area.
>
>Contact Tonia Breckenridge, Event Coordinator at (850) 469-2353. Flyers
will
>be mailed this week. E-mail at TABrecken@aol.com
>
>- --part0_919987591_boundary--
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 18:32:03 -0600
>From: ODDBALSTER@aol.com
>Subject: Slide "Plains"
>
>Hello Histonetters, I was hoping you could help me a couple problems.My
>pathologist was complaining that the stained H & E sections in our daily
work
>was somewhat on different "plains" and needed constant focusing while under
>the scope. I sat down with her and went over some slides, these sections
were
>somewhat "fuzzy or hazy" in some places and crisp in other, which makes me
>think possibly it's the mounting medium...we've been using this medium for
>sometime (resinous) and unfortunately we're on contract so my options are
>limited (for purchasing that is). Could it possibly be the deparaffination
>process? We microwave our sections to melt the paraffin, and put them in
>Xylene for 6 minutes to clear before sending them down the stainer. It does
>not happen on all the tissue, and there does not seem to be any preference
as
>to type of specimen either (happens to bx's and surgicals). Any
suggestions?
>Also, we seem to be getting holes in our blocks after embedding, the VERY
>irritating experience is becoming more and more frequent. We have fresh
>paraffin in our dispenser (and processor) and have a cold plate at optimal
>temperature, yet it seems at least 4-5 blocks per day are "breaking" when
>trimming because of these holes. Is it possible that we may be embedding
too
>fast? Not giving the warm paraffin time to combine with the cold paraffin
>after inserting the tissue in the mold? Could it be that we aren't cleaning
>the molds enough? Again, any suggestions? Thanks for your help in advance,
man
>I love this net......
>
>Kari Zajic HT,MLT
>Lead Histotech
>Florida
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 19:10:44 -0600
>From: R Clarke <rr_clarke@bc.sympatico.ca>
>Subject: GROCOTT
>
>Hi,
>
>Does anyone have a method for Grocott without using Chromic Acid?
>Thanks!
>
>Rose Clarke
>Burnaby Hospital
>
>
>
>----------------------------------------------------------------------
>
>Date: 25 Feb 1999 20:15:27 -0600
>From: "Aidan C. Schurr" <aidan.schurr@stonebow.otago.ac.nz>
>Subject: Re: Slide "Plains"
>
>Kari,
>Just a couple of suggestions - the different planes you describe are quite
>possibly due to different thicknesses of the mountant under the coverslip.
>Too much mountant leads to a different refractive index, which can give the
>problem you describe. Not too sure with the haziness - could be the same
>problem if the mountant layer is **very** thick, but this seems unlikely.
>Another possibilty is that you have water in your zylene, or dehydration
>has been incomplete. You could perhaps try using two changes of xylene.
>
>Hope this is of some help
>
>Good luck
>Aidan, New Zealand
>
>
>>Hello Histonetters, I was hoping you could help me a couple problems.My
>>pathologist was complaining that the stained H & E sections in our daily
work
>>was somewhat on different "plains" and needed constant focusing while
under
>>the scope. I sat down with her and went over some slides, these sections
were
>>somewhat "fuzzy or hazy" in some places and crisp in other, which makes me
>>think possibly it's the mounting medium...we've been using this medium for
>>sometime (resinous) and unfortunately we're on contract so my options are
>>limited (for purchasing that is). Could it possibly be the deparaffination
>>process? We microwave our sections to melt the paraffin, and put them in
>>Xylene for 6 minutes to clear before sending them down the stainer. It
does
>>not happen on all the tissue, and there does not seem to be any preference
as
>>to type of specimen either (happens to bx's and surgicals). Any
suggestions?
>>Also, we seem to be getting holes in our blocks after embedding, the VERY
>>irritating experience is becoming more and more frequent. We have fresh
>>paraffin in our dispenser (and processor) and have a cold plate at optimal
>>temperature, yet it seems at least 4-5 blocks per day are "breaking" when
>>trimming because of these holes. Is it possible that we may be embedding
too
>>fast? Not giving the warm paraffin time to combine with the cold paraffin
>>after inserting the tissue in the mold? Could it be that we aren't
cleaning
>>the molds enough? Again, any suggestions? Thanks for your help in advance,
>man
>>I love this net......
>>
>>Kari Zajic HT,MLT
>>Lead Histotech
>>Florida
>
>
>/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/
> Aidan C. Schurr BMedLabSc
> Histology Service Unit ++64 3 4797152
> Pathology Department
> Dunedin School of Medicine
>
> acschurr@bigfoot.com
> aidan.schurr@stonebow.otago.ac.nz
>\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
>
>
>
>
>Here are the messages received yesterday!
>
>
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