Re: Antibody storage (temperature, time)
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
You'd think the immunologists would have sorted out optimal
storage conditions years ago. I have talked to a few local
ones, and they work by "common sense" and hearsay:
avoid repeated freezing/thawing; use -80; -20 is OK;
+4 and throw out in 6 months; etc. No consistency at all.
A general suspicion exists of suppliers' expiration dates,
which might be contrived to make us buy more often.
Has anyone seen a published comparative study, in a
regular peer-reviewed journal, of the effects of
different storage conditions on different kinds
of antibodies?
John Kiernan, London, Canada.
On Mon, 22 Feb 1999 rschoonh@sph.unc.edu wrote:
> I have been using antibodies that were made for us ~12 years ago which were
> aliquated and stored in one of our -80 freezes and they still work
> perfectly. Don't know about storing them at -196, I would doubt that it
> would hurt them but I don't think that it would extend their usfullness.
>
> I do know that when these same antibodies were stored at -20 (some were put
> in the -20 by a predecessor) they started to lose potency after about 3
> years. That was when I 'discovered' the ones stored in the -80.
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