RE: chrome gelatin
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
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Content-Type: | text/plain; charset="us-ascii" |
On Fri, 19 Feb 1999, Gayle Callis wrote:
> 5gm gelatin in 1 liter distilled water, add 1 gm
> chromium potassium sulfate
I'd like to add that it's best to dissolve the ingredients
in separate lots of water and then combine the solutions.
Otherwise you can end up with insoluble lumps of the
Cr-gelatin complex, with most of the solution not
"chromed." Once made, you cannot dilute it further,
so make either a dilute version for the water bath
(e.g. 0.1% gelatin, 0.05% chrome alum) or a stronger
one for smearing onto slides with the finger-tip
(1%:0.1%). The intermediate strength (0.5%:0.2-0.5%)
is good for dipping slides then drying.
These solutions do not keep well - they fill up with
fungi after a few days. Once I aliquoted some 1%:0.1%
chrome gelatin into injection ampoules, sealed them and
autoclaved. These kept well, and I went on using them
for over a year by the digital smearing method. No
loss of adhesive power, but it was much more trouble than
making a fresh solution every time!
I use slides subbed with chrome gelatin for nearly
everything. True, the gelatin layer does become
coloured, but never strongly enough to interfere
with examining the stained sections.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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