Cindy,
I have a receipe that I learned about at the NSH meeting in Salt Lake city
last year.
	Citrate buffer pH 6.0
Citric acid 0.1M
    citric acid monohydrate.............. 21.01 gm
     distilled water........................ 1000 mL

Sodium Citrate 0.1M
     sodium citrate dihydrate............. 29.41 gm
      distilled water........................ 1000 mL

Working Solution
      0.1M citric acid.................. 9.0 mL
      0.1M sodium citrate.......... 41.0 mL
      distilled water.................. 450.0 mL

adjust the pH with either 1N sodium hydroxide or 1N hydrochloric acid.

This is good for general use.  However, some antibodies require a higher pH
buffer.  It's trial and error Hope this helps.

Joe Nocito, B.S., HT(ASCP)QIHC
Histology Supervisor
Christus Santa Rosa Hospitals
San Antonio, Texas

> -----Original Message-----
> From:	vandeplas@aurion.nl [SMTP:vandeplas@aurion.nl]
> Sent:	Wednesday, February 24, 1999 6:59 PM
> To:	Histonet@Pathology.swmed.edu
> Subject:	Re: antigen retrival
>
> >Cindy wrote:
> >    I am going to be using microwave antigen retrival for a couple of
> >antibodies that I have not previously stained with. I was wondering
> >if anyone had any recipes for Citrate Buffers or any other
> >buffer solutions used for this purpose?
> >
> -----------------------------------
> Dear Cindy,
> The optimal AR protocol differs from primary  to primary.
> The review article by Shan-Rong Shi might be of help.
> Shi et al (1997) J. Histochem. Cytochem. 45, 327-343
> Peter
>
> ========================================
> Peter van de Plas
> AURION
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> 6702 AA Wageningen
> The Netherlands
> phone: (31)-317-497676
> fax: (31)-317-415955
> http://www.aurion.nl
>
>