RE: Formalin/Alcohol Question
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From: | "Nocito, Joseph" <joseph_nocito@srhc.iwhs.org> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
John,
our experience with formalin substitutes such as alcoholic formalin and zenk
formalin have not been good. We have a tendency to get increased
non-specific background staining.
> -----Original Message-----
> From: John Spair [SMTP:jspair@multicare.com]
> Sent: Thursday, February 25, 1999 12:10 PM
> To: histonet@pathology.swmed.edu
> Subject: Fw: Formalin/Alcohol Question
>
>
> Histonetters:
>
> Some people in pathology say they have experienced a loss of
> immunoreactivity when tissues are fixed in formol-alcohol. One lab for
> instance that we have referred out ICC, won't do ICC on any tissue fixed
> in
> formol-alcohol. Another lab says they have no trouble whatsover.
> Recently
> our pathologists started fixing our breast and colon cases in
> formol-alcohol
> and since most of these breast cases get a "breast battery" panel done on
> them, such as ER/PR, etc. - a concern has arisen between our pathology
> group
> about the use of formol-alcohol.
>
> I'm curious to find out other histonetters opinions, or experiences before
> making a final decision and telling our pathology group that we cannot use
> it any longer. Thank you.
>
>
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