RE: Formalin/Alcohol Question

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From:"Nocito, Joseph" <> (by way of histonet)
To:histonet <>
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our experience with formalin substitutes such as alcoholic formalin and zenk
formalin have not been good.  We have a tendency to get increased
non-specific background staining.

> -----Original Message-----
> From:	John Spair []
> Sent:	Thursday, February 25, 1999 12:10 PM
> To:
> Subject:	Fw: Formalin/Alcohol Question
> Histonetters:
> Some people in pathology say they have experienced a loss of
> immunoreactivity when tissues are fixed in formol-alcohol.  One lab for
> instance that we have referred out ICC, won't do ICC on any tissue fixed
> in
> formol-alcohol.  Another lab says they have no trouble whatsover.
> Recently
> our pathologists started fixing our breast and colon cases in
> formol-alcohol
> and since most of these breast cases get a "breast battery" panel done on
> them, such as ER/PR, etc. - a concern has arisen between our pathology
> group
> about the use of formol-alcohol.
> I'm curious to find out other histonetters opinions, or experiences before
> making a final decision and telling our pathology group that we cannot use
> it any longer.  Thank you.

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