RE: CMV in-situ

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Dr Kwan:

We have several new in-situ probes available and are currently beta site
testing room temperature in-situ that can run automated on our Optimax
instrument.  Are you interested in talking about these?  Dr Max Loda @
The Farber is working with us to try to develop the best probes possible.
 I think you know Dr Loda and his reputation.

Have a great Friday!

Lisa Jensen-Long

 -----Original Message-----
From: paul.kwan []
Sent: Thursday, February 18, 1999 9:53 PM
Subject: CMV in-situ


I have been quite successful with Kreatech's CMV ISH kit, now being
distributed by Zymed.

I use the digoxigenin labelled probe.

Instead of digesting with pepsin as suggested by the manufacturer, I use
protease K (100 microgram per ml protease K type XXVIII, Sigma P-4914)
for 10
minutes at 37 degrees C.

Good luck,

Paul Kwan, Ph.D.
New England Medical Center

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Date: Thu, 18 Feb 1999 17:15:46 -0500 (EST)
From: Paul Kwan <>
Subject: CMV in-situ
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