RE: CMV in-situ
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From: | lisajl@biogenex.com (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
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Dr Kwan:
We have several new in-situ probes available and are currently beta site
testing room temperature in-situ that can run automated on our Optimax
instrument. Are you interested in talking about these? Dr Max Loda @
The Farber is working with us to try to develop the best probes possible.
I think you know Dr Loda and his reputation.
Have a great Friday!
Lisa Jensen-Long
Biogenex
-----Original Message-----
From: paul.kwan [mailto:paul.kwan@es.nemc.org]
Sent: Thursday, February 18, 1999 9:53 PM
To: LMARGRAF@childmed.dallas.tx.us
Subject: CMV in-situ
Hi,
I have been quite successful with Kreatech's CMV ISH kit, now being
distributed by Zymed.
I use the digoxigenin labelled probe.
Instead of digesting with pepsin as suggested by the manufacturer, I use
protease K (100 microgram per ml protease K type XXVIII, Sigma P-4914)
for 10
minutes at 37 degrees C.
Good luck,
Paul Kwan, Ph.D.
New England Medical Center
Boston
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Date: Thu, 18 Feb 1999 17:15:46 -0500 (EST)
From: Paul Kwan <paul.kwan@es.nemc.org>
Subject: CMV in-situ
To: LMARGRAF@childmed.dallas.tx.us
Cc: histonet@Pathology.swmed.edu
Message-id: <vines.3u49+E27nqA@nemc-ese.ban.nemc.org>
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