IHC titer

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From:Verrucous@aol.com (by way of histonet)
To:histonet <histonet@magicnet.net>
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Hi Histoinans !
   I would like some help with titering my immunohistochemical primary
antibodies, please.
   How many dilutions and in what ranges. What does an economical pipette for
this procedure cost? Do you use titer records & retain titer slides? Do you
adjust antibodies on special request from the Pathologist to demonstrate
specifically “hot” markers?
   I’ve told my Pathologists that we need to invest in the pipette for proper
titering procedure. I keep records of the dilutions we arrive at and make the
Pathologist sign off on each lot of primary antibody.
   Thank You for any help

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