IHC on hard tissue

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From:RUSS ALLISON <Allison@cardiff.ac.uk> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Dear all (particularly boneheads),

One of our researchers wants to:-

do immunocytochemistry on thin, gound (undecalcified) pieces of
tooth! (can you believe that?). They will be approx. 150 microns
thick.  The antibody will be to a naturally occuring protein.

Any help, experience and (most) suggestions welcomed.  Make that
"begged".

Many thanks in advance,

Diolch yn fawr

Russ Allison, Wales




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