Fluorescent bone labeling

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From:Angel Duty <gt4451b@prism.gatech.edu> (by way of histonet)
To:histonet <histonet@magicnet.net>
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I am currently during in vivo rabbit studies of bone remodelling.  My
biopsies are of
well-mineralized trabecular bone measuring 1/4" in diameter and 1/4" in
length. I
am just beginning to include in my protocol oxytetracycline and calcein
injections to label
new bone formation prior to biopsy. It has been suggested to me that
fixation in 70%
ethanol and methyl methacrylate embedding will preserve the fluorescent
dye better than
10% formalin fixation and decalcified paraffin embedded sections (which
is our normal
method for processing the bone samples).  Has anyone found one avenue to
be  better
than the other?

Thank you for your help.

Angel Duty
Emory/Georgia Tech Biomedical Engineering

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