Antibody dilution.

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From:Ian Montgomery <ian.montgomery@bio.gla.ac.uk> (by way of histonet)
To:histonet <histonet@magicnet.net>
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	Just bought , from Chemicon, a mouse anti-human collagen type VI
monoclonal antibody (Cat.No. MAB3303). If anyone is using it, what dilution
are you using, a ball park figure will do. I asked Chemicon and they say
start at 1:250 for brightfield and 1:20 for fluorescence. I'll be using the
antibody for a confocal study so must use fluorescence. By the time I do a
dilution trial starting at the suggested 1:20 and with only 50Ál of neat
antibody the study is going to cost a fortune. Any ideas on dilution will
be most welcome.
Ian.

Dr. Ian Montgomery,
West Medical Building,
University of Glasgow,
Glasgow,
G12 8QQ,
Scotland.
Tel: 0141 339 8855 Extn. 6602.
Fax: 0141 330 4100.
e-mail: ian.montgomery@bio.gla.ac.uk




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